Recombinant Human Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G, His-tagged

• Cat.No.: PPM1G-1756H
• Product Overview: Recombinant humanPPM1G protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Cat. No.: PPM1G-1756H
• Description: PPM1G, as known asprotein phosphatase 1G, is a member of the PP2C family of Ser/Thr proteinphosphatases that are negative regulators of cell stress response pathways.This enzyme localizes to the cytoplasm and is widely expressed, with mostabundant expression detected in testis, skeletal muscle and heart. It isnecessary for the dephosphorylation of pre-mRNA splicing factors, which is animportant process for the formation of the functional spliceosome.
• Concentration: 1 mg/ml
• Source: Human
• Host: E.coli
• Form: Supplied as a liquidin 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl, 1mM DTTand 0.1mM PMSF.
• Purity: > 85% by SDS -PAGE
• Sequence: 1-546 amino acids:MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELDSETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGRPTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEAGPEDS
• Molecular Mass: 61.4 kDa (566aa)
• Applications: SDS-PAGE
• Storage: Store at 4 deg C forshort term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg Cfor long term storage. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: PPM1G

Gene Information

• Gene Name: PPM1Gprotein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform [ Homosapiens ]
• Synonyms: PPM1G; proteinphosphatase, Mg2+/Mn2+ dependent, 1G; PPM1C; PP2CG; PPP2CG; MGC1675; MGC2870;PP2CGAMMA; PP2Cgamma; PP2C-gamma; PP2C, gamma; EC 3.1.3.16;OTTHUMP00000123426; protein phosphatase 1G; Protein phosphatase 1G; proteinphosphatase 1C; protein phosphatase 2C gamma isoform; protein phosphatase 2Cisoform gamma; Protein phosphatase 2C isoform gamma; Protein phosphatasemagnesium-dependent 1 gamma; protein phosphatase magnesium-dependent 1 gamma;protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase1G (formerly 2C), magnesium-dependent, gamma isoform
• Gene ID: 5496
• mRNA Refseq: NM_002707
• Protein Refseq: NP_002698
• MIM: 605119
• UniProt ID: O15355
• Chromosome Location: 2p23.3
• Pathway: mRNA processing
• Function: proteinserine/threonine phosphatase activity; manganese ion binding; magnesium ionbinding; hydrolase activity

Reviews

11/14/2022

PPM1G protein's utility in protein electron microscopy structure analysis is commendable.

12/03/2019

It enables researchers to delve deeper into the intricacies of protein behavior and aids in advancing our knowledge in various fields, such as molecular biology, biochemistry, and structural biology.

11/11/2019

It demonstrates excellent performance in ELISA assays, making it an ideal choice for researchers studying protein-protein interactions, antibody detection, or biomarker identification.

Q&As

Can PPM1G be targeted for cancer treatment?

Research is ongoing to explore the therapeutic potential of targeting PPM1G in cancer. Inhibiting or modulating its activity may be a strategy for cancer therapy.

Are there any known associations between PPM1G and cancer?

Yes, abnormal expression of PPM1G has been linked to certain cancers, suggesting its potential role as a biomarker or therapeutic target.

Are there any genetic disorders associated with PPM1G mutations?

While rare, mutations in the PPM1G gene have been reported and may be associated with certain genetic disorders. Further research is needed to understand the implications.

How does PPM1G contribute to DNA repair mechanisms?

PPM1G is involved in the regulation of DNA repair processes, influencing the repair of damaged DNA and maintaining genomic stability.

Can PPM1G be used as a diagnostic marker for specific diseases?

PPM1G expression levels may serve as a diagnostic marker for certain diseases, including some cancers, but more studies are required to establish its diagnostic utility.