Recombinant Human PSMB5 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PSMB5-6693H
• Product Overview: PSMB5 MS Standard C13 and N15-labeled recombinant protein (NP_002788) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 28.5 kDa
• AA Sequence: MALASVLERPLPVNQRGFFGLGGRA DLLDLGPGSLSDGLSLAAPGWGVPE EPGIEMLHGTTTLAFKFRHGVIVAA DSRATAGAYIASQTVKKVIEINPYL LGTMAGGAADCSFWERLLARQCRIY ELRNKERISVAAASKLLANMVYQYK GMGLSMGTMICGWDKRGPGLYYVDS EGNRISGATFSVGSGSVYAYGVMDR GYSYDLEVEQAYDLARRAIYQATYR DAYSGGAVNLYHVREDGWIRVSSDN VADLHEKYSGSTPTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PSMB5 proteasome 20S subunit beta 5 [ Homo sapiens (human) ]
• Official Symbol: PSMB5
• Synonyms: PSMB5; proteasome (prosome, macropain) subunit, beta type, 5; proteasome subunit beta type-5; MB1; X; proteasome chain 6; proteasome subunit X; proteasome subunit MB1; macropain epsilon chain; proteasome epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome subunit, beta type, 5; PSX large multifunctional protease X; multicatalytic endopeptidase complex epsilon chain; LMPX; MGC104214
• Gene ID: 5693
• mRNA Refseq: NM_002797
• Protein Refseq: NP_002788
• MIM: 600306
• UniProt ID: P28074