Recombinant Human RAF1 Protein, His-tagged

• Cat.No.: RAF1-19H
• Product Overview: Human Ras Binding Domain (RBD) of Raf-1 Proto-Oncogene, Serine/Threonine Kinase (RAF1), (UniProt # P04049, amino acids 1-149), expressed with an N-terminal 6XHis and glutathione S-transferase (GST) dual tag in E. coli. The dual 6xHis-GST tag allows immobilization of Raf1 RBD Protein and RBD-Ras complexes on either Ni-NTA or glutathione agarose resin columns.

Specification

• Description: The Raf1 RBD protein binds specifically to GTP-bound Ras proteins. Ras proteins, like other small GTPases, regulate molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ras binds specifically to the Ras-binding domain (RBD) of Raf1 to control downstream signaling cascades. The affinity between RBD and GDP-bound Ras is three orders of magnitude lower. Therefore, the RBD protein can be used to specifically precipitate active, GTP-bound Ras as well as to specifically block the activity of Ras in vitro and in vivo.
• Source: E. coli
• Species: Human
• Tag: His
• Purity: > 80% by Coomassie staining
• Applications: Measurement of the GTP/GDP bound ratio of Ras in vitro.
  Quantitation of GTP-bound Ras from tissue and tissue culture cell lysates.
  RAS/RAF binding studies.
  Inhibitor screening for RAS/RAF interaction
• Notes: This product is for laboratory research use only.
• Storage: Storage is recommended at -20 centigrade for longer periods of time.
• Concentration: 1.0 mg/mL by A280
• Storage Buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, and 20% Glycerol.
• Shipping: Product requires shipping on ice packs.

Gene Information

• Gene Name: RAF1 Raf-1 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: RAF1
• Synonyms: RAF1; Raf-1 proto-oncogene, serine/threonine kinase; NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; C-Raf proto-oncogene, serine/threonine kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; EC 2.7.11.1
• Gene ID: 5894
• mRNA Refseq: NM_002880
• Protein Refseq: NP_002871
• MIM: 164760
• UniProt ID: P04049