Recombinant Human RASA1 293 Cell Lysate

• Cat.No.: RASA1-2510HCL

Specification

• Description: Antigen standard for RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RASA1 RAS p21 protein activator (GTPase activating protein) 1 [ Homo sapiens ]
• Official Symbol: RASA1
• Synonyms: RASA1; RAS p21 protein activator (GTPase activating protein) 1; RASA; ras GTPase-activating protein 1; capillary malformation arteriovenous malformation; CM AVM; GAP; p120GAP; p120RASGAP; triphosphatase-activating protein; PKWS; CMAVM; CM-AVM; RASGAP; DKFZp434N071
• Gene ID: 5921
• mRNA Refseq: NM_002890
• Protein Refseq: NP_002881
• MIM: 139150
• UniProt ID: P20936
• Chromosome Location: 5q13
• Pathway: Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem
• Function: GTPase binding; Ras GTPase activator activity; glycoprotein binding; potassium channel inhibitor activity; protein binding; receptor binding

Reviews

10/28/2019

The manufacturer's reliable quality allows me to conduct experiments with confidence.

09/01/2018

This protein reagent exhibits impeccable performance, providing stable and accurate results for every experiment.

07/09/2018

I am grateful for the outstanding performance and stable quality of this protein substance, which has greatly facilitated my research work.