Recombinant Human RB1 protein

• Cat.No.: RB1-195H
• Product Overview: Recombinant Human RB1 protein was expressed in Escherichia coli.

Specification

• Description: Rb encoded by the RB1 gene in humans, is expressed by retina and belongs to the etinoblastoma-associated protein family. The hole protein consists of 928 a.a. and the rHuRb fragment occupies sequence of 792-929 a.a.. Rb is a key regulator of entry into cell division that acts as a tumor suppressor. It has many functions, for example, promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C, and acts as a transcription repressor of E2F1 target genes and so on. The rHuRb is the region that rich of modified residue like phosphothreonine and N6-acetyllysine.
• Source: E.coli
• Species: Human
• Form: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
• Molecular Mass: Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
• Protein length: 146
• AA Sequence: MASFPSSPLRIPGGNIYISPLKSPY KISEGLPTPTKMTPRSRILVSIGES FGTSEKFQKINQMVCNSDRVLKRSA EGSNPPKPLKKLRFDIEGSDEADGS KHLPGESKFQQKLAEMTSTRTRMQK QKMNDSMDTSNKEEKHHHHHH
• Endotoxin: Less than 1 EU/μg of rHuRb137, His as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: RB1
• Official Symbol: RB1
• Synonyms: RB1; retinoblastoma 1; OSRC, osteosarcoma; retinoblastoma-associated protein; RB; retinoblastoma suspectibility protein; pRb; OSRC; pp110; p105-Rb
• Gene ID: 5925
• mRNA Refseq: NM_000321
• Protein Refseq: NP_000312
• MIM: 614041
• UniProt ID: P06400

Reviews

07/15/2022

Efficient protein purification, delivers consistent yield and purity.

01/19/2021

Expertise in protein engineering, a trusted resource for research.

10/09/2019

Reliable protein crystallization services, exceptional results achieved.

Q&As

What is the impact of RB1 mutations on the development of retinoblastoma and other cancers?

Mutations in RB1 lead to retinoblastoma and increase the risk of other cancers, disrupting normal cell cycle control.

How does RB1 interact with transcription factors and affect gene expression?

RB1 interacts with transcription factors to regulate gene expression, impacting various cellular processes.

What role does RB1 play in DNA damage response and repair mechanisms?

RB1 plays a crucial role in the DNA damage response, facilitating repair and maintaining genomic stability.

How does RB1 regulate the cell cycle, particularly the transition from G1 to S phase?

RB1 controls the G1 to S phase transition in the cell cycle, preventing unregulated cell division.

How do alterations in RB1 expression or function influence stem cell differentiation and tissue regeneration?

Changes in RB1 function affect stem cell differentiation and tissue regeneration, influencing tissue maintenance and repair.

What are the mechanisms by which RB1 modulates cellular senescence and apoptosis?

RB1 influences cellular senescence and apoptosis, playing a role in aging and cell turnover.

How does RB1 contribute to tumor suppression and the prevention of uncontrolled cell proliferation?

As a tumor suppressor, RB1 prevents excessive cell proliferation and contributes to cancer prevention.