Recombinant Human RET Protein, His-tagged

• Cat.No.: RET-2652H
• Product Overview: Recombinant Human RET Protein (Thr78-Phe272) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.3 kDa
  Accurate Molecular Mass: 26 kDa
• Protein length: Thr78-Phe272
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: RET ret proto-oncogene [ Homo sapiens (human) ]
• Official Symbol: RET
• Synonyms: RET; ret proto-oncogene; Hirschsprung disease 1, HSCR1, MEN2A, MEN2B, MTC1, multiple endocrine neoplasia and medullary thyroid carcinoma 1; proto-oncogene tyrosine-protein kinase receptor Ret; cadherin related family member 16; CDHF12; CDHR16; PTC; RET51; proto-oncogene c-Ret; receptor tyrosine kinase; RET transforming sequence; cadherin family member 12; hydroxyaryl-protein kinase; cadherin-related family member 16; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease); MTC1; HSCR1; MEN2A; MEN2B; RET-ELE1
• Gene ID: 5979
• mRNA Refseq: NM_020630
• Protein Refseq: NP_065681
• MIM: 164761
• UniProt ID: P07949