Recombinant Human Retinoblastoma Binding Protein 4, His-tagged

• Cat.No.: RBBP4-6964H
• Product Overview: Recombinant human RBBP4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: Retinoblastoma binding protein 4, also known as RBBP4, is a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. RBBP4 is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM EDTA.
• Molecular Mass: 50.2 kDa (449aa)
• Protein length: 1-425aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMADKEA AFDDAVEERV INEEYKIWKK NTPFLYDLVM THALEWPSLT AQWLPDVTRP EGKDFSIHRL VLGTHTSDEQ NHLVIASVQL PNDDAQFDAS HYDSEKGEFG GFGSVSGKIE IEIKINHEGE VNRARYMPQN PCIIATKTPS SDVLVFDYTK HPSKPDPSGE CNPDLRLRGH QKEGYGLSWN PNLSGHLLSA SDDHTICLWD ISAVPKEGKV VDAKTIFTGH TAVVEDVSWH LLHESLFGSV ADDQKLMIWD TRSNNTSKPS HSVDAHTAEV NCLSFNPYSE FILATGSADK TVALWDLRNL KLKLHSFESH KDEIFQVQWS PHNETILASS GTDRRLNVWD LSKIGEEQSP EDAEDGPPEL LFIHGGHTAK ISDFSWNPNE PWVICSVSED NIMQVWQMAE NIYNDEDPEG SVDPEGQGS
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RBBP4 retinoblastoma binding protein 4 [ Homo sapiens ]
• Official Symbol: RBBP4
• Synonyms: RBBP4; retinoblastoma binding protein 4; histone-binding protein RBBP4; NURF55; RbAp48; RBBP-4; CAF-I p48; CAF-1 subunit C; CAF-I 48 kDa subunit; MSI1 protein homolog; retinoblastoma-binding protein 4; retinoblastoma-binding protein p48; chromatin assembly factor 1 subunit C; chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; nucleosome-remodeling factor subunit RBAP48; RBAP48
• Gene ID: 5928
• mRNA Refseq: NM_001135255
• Protein Refseq: NP_001128727
• MIM: 602923
• UniProt ID: Q09028
• Chromosome Location: 1p35.1
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Deposition of New CENPA-containing Nucleosomes at the Centromere, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; G0 and Early G1, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem
• Function: contributes_to DNA-dependent ATPase activity; histone binding; histone deacetylase binding; protein binding

Reviews

12/20/2020

They can offer specialized formulations, concentrations, or modifications of RBBP4 protein to suit unique experimental requirements.

04/19/2018

The manufacturer can further support researchers by providing customized solutions tailored to their specific needs.

06/20/2016

Collaborative discussions with the manufacturer can also help optimize experimental protocols and provide insights into the best practices in working with RBBP4.

Q&As

How is RBBP4 involved in cell differentiation?

RBBP4 participates in the regulation of genes involved in cell differentiation, which is crucial for the development and maintenance of tissues.

Are there any clinical applications for RBBP4 in cancer treatment?

Research into targeting RBBP4 in cancer therapy is ongoing, as it may help in developing novel treatments for certain types of cancer.

How can RBBP4 influence cancer progression?

RBBP4's role in gene regulation can impact the expression of genes involved in cell proliferation and differentiation, affecting cancer progression.

Can RBBP4 be used as a diagnostic marker for cancer?

RBBP4 is not typically used as a standalone diagnostic marker, but its expression levels can be studied to understand the molecular mechanisms underlying cancer.

What other diseases or conditions are associated with RBBP4?

RBBP4's role in gene regulation implicates it in various diseases and conditions beyond cancer, including neurological disorders and developmental diseases.