Recombinant Human RFK Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RFK-1608H
• Product Overview: RFK MS Standard C13 and N15-labeled recombinant protein (NP_060809) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 18.4 kDa
• AA Sequence: MPRADCIMRHLPYFCRGQVVRGFGR GSKQLGIPTANFPEQVVDNLPADIS TGIYYGWASVGSGDVHKMVVSIGWN PYYKNTKKSMETHIMHTFKEDFYGE ILNVAIVGYLRPEKNFDSLESLISA IQGDIEEAKKRLELPEHLKIKEDNF FQVSKSKIMNGHTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RFK riboflavin kinase [ Homo sapiens (human) ]
• Official Symbol: RFK
• Synonyms: RFK; riboflavin kinase; FLJ11149; RIFK; flavokinase; 0610038L10Rik; ATP:riboflavin 5-phosphotransferase; RP11-422N19.2
• Gene ID: 55312
• mRNA Refseq: NM_018339
• Protein Refseq: NP_060809
• MIM: 613010
• UniProt ID: Q969G6

Reviews

01/13/2020

I highly recommend the RFK protein for various experimental applications.

03/26/2018

It has demonstrated outstanding performance in ELISA, providing reliable and precise results.

08/29/2017

It has been invaluable in protein electron microscopy structure analysis, facilitating detailed investigations and yielding valuable insights.

Q&As

Can RFK protein be a therapeutic target in drug development?

Yes, RFK protein modulation may hold potential for therapeutic intervention in diseases related to its deficiency or dysregulation.

In what other areas of medicine is RFK protein relevant?

RFK protein's involvement in cellular metabolism makes it relevant in fields such as oncology, neurology, and mitochondrial disorders.

Are there any known side effects or risks associated with RFK protein-targeted therapies?

Generally, riboflavin supplementation is considered safe; however, high doses might lead to minor side effects like diarrhea or urine discoloration.

What are the treatment approaches for RFK protein-related disorders?

Treatment primarily involves riboflavin supplementation, aiming to restore adequate levels of active FMN and address the underlying deficiency.

How does the understanding of RFK protein aid in personalized medicine?

Understanding RFK protein variants or deficiencies helps in tailoring riboflavin supplementation and treatments for individuals with specific genetic profiles or conditions.