Recombinant Human RGN 293 Cell Lysate

• Cat.No.: RGN-2388HCL

Specification

• Description: Antigen standard for regucalcin (senescence marker protein-30) (RGN), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RGN regucalcin (senescence marker protein-30) [ Homo sapiens ]
• Official Symbol: RGN
• Synonyms: RGN; regucalcin (senescence marker protein-30); regucalcin; RC; senescence marker protein 30; SMP30; SMP-30; gluconolactonase; senescence marker protein-30; GNL
• Gene ID: 9104
• mRNA Refseq: NM_004683
• Protein Refseq: NP_004674
• MIM: 300212
• UniProt ID: Q15493
• Chromosome Location: Xp11.3
• Pathway: Ascorbate and aldarate metabolism, organism-specific biosystem; Ascorbate and aldarate metabolism, conserved biosystem; Ascorbate biosynthesis, animals, glucose-1P =>ascorbate, organism-specific biosystem; Ascorbate biosynthesis, animals, glucose-1P => ascorbate, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: calcium ion binding; calcium ion binding; enzyme regulator activity; gluconolactonase activity; hydrolase activity; zinc ion binding

Reviews

12/27/2020

Precise results, highly recommended for research.

11/09/2019

Efficient service, great for protein analysis.

06/12/2017

Accurate data, crucial for our experiments.

Q&As

What role does RGN protein play in tissue regeneration and repair?

RGN plays a key role in tissue repair and regeneration, potentially by promoting cell differentiation and proliferation.

What factors regulate the expression and activity of RGN protein?

Both exogenous and endogenous factors can regulate RGN's expression and activity, including environmental stress and drug interactions.

How does RGN protein expression vary across different types of cancer?

RGN expression varies in different cancers, reflecting its complex role in cancer development.

What physiological processes are affected by heterogeneous expression of RGN protein?

Different levels of RGN expression impact cellular growth regulation, metabolic balance, and differentiation processes.

How do structural changes in RGN protein affect its biological function?

Minor structural changes can significantly alter RGN's activity, affecting its biological function.

How does RGN protein interact with other proteins to affect cell signaling?

RGN influences various intracellular signaling pathways through interactions with other proteins.

What is the effect of RGN protein on cell proliferation and apoptosis?

RGN can either promote or inhibit cell proliferation and apoptosis by modulating specific signaling pathways.