Recombinant Human ribosomal protein S2, His-tagged

• Cat.No.: RPS2-306H
• Product Overview: RPS2, 1-293aa, Human, His tag, E.coli

Specification

• Description: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS2 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S5P family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with mouse LLRep3. It is co-transcribed with the small nucleolar RNA gene U64, which is located in its third intron. Recombinant human RPS2 protein, fused to His-tag at N-terminus, was expressed in E.coli .
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
• Molecular Mass: 33.7 kDa (316aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADDAGA AGGPGGPGGP GMGNRGGFRG GFGSGIRGRG RGRGRGRGRG RGARGGKAED KEWMPVTKLG RLVKDMKIKS LEEIYLFSLP IKESEIIDFF LGASLKDEVL KIMPVQKQTR AGQRTRFKAF VAIGDYNGHV GLGVKCSKEV ATAIRGAIIL AKLSIVPVRR GYWGNKIGKP HTVPCKVTGR CGSVLVRLIP APRGTGIVSA PVPKKLLMMA GIDDCYTSAR GCTATLGNFA KATFDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT
• Purity: >85 % by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RPS2 ribosomal protein S2 [ Homo sapiens ]
• Official Symbol: RPS2
• Synonyms: RPS2; ribosomal protein S2; 40S ribosomal protein S2; LLREP3; S2; OK/KNS-cl.6; protein LLRep3; 40S ribosomal protein S4; MGC102851; MGC117344; MGC117345
• Gene ID: 6187
• mRNA Refseq: NM_002952
• Protein Refseq: NP_002943
• MIM: 603624
• UniProt ID: P15880
• Chromosome Location: 16p13.3
• Pathway: Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; Disease, organism-specific biosystem; Eukaryotic Translation Elongation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Eukaryotic Translation Termination, organism-specific biosystem
• Function: RNA binding; fibroblast growth factor 1 binding; fibroblast growth factor 3 binding; protein binding; structural constituent of ribosome

Reviews

05/18/2023

The catalytic efficiency is high, the time is short, and the time is saved.

04/26/2021

The quality I use is so good that I have already recommended this product to my colleagues and friends.

11/22/2020

It has high sensitivity and strong specificity.

Q&As

What are the effects of mutations in RPS2 on cells?

Mutations in RPS2 may have a variety of effects on cells, such as affecting protein synthesis, leading to apoptosis, and inducing autophagy. These effects may be related to the occurrence of some diseases, such as cancer, neurodegenerative diseases, etc.

Why should information from RPS2 be used to guide diagnosis or treatment in clinical practice?

In clinical practice, the information of RPS2 can be used to guide the diagnosis and treatment of diseases. For example, by detecting RPS2 expression levels or mutations, doctors can be able to diagnose and assess the prognosis of certain diseases, and treatment strategies for RPS2 can also provide patients with more effective treatment options.

How to study the interaction network of RPS2?

The interaction network of RPS2 can be studied using molecular biology and biochemical methods, such as yeast two-hybrid, affinity chromatography, mass spectrometry, etc. These methods can help us understand the function and mechanism of action of RPS2 in cells.

How do I set up a cell model of RPS2 overexpression or knockout?

RPS2 overexpression or knockout cell models can be established by gene transfection. The vector of the gene of interest or a specific knockout gene is introduced into the cells, and the stably expressed or knocked out cell line is obtained through screening and identification.

How does RPS2 interact with other proteins or molecules?

The protein interacts with other ribosomal proteins and translation factors and participates in the protein synthesis process. In addition, it may interact with molecules in some signal transduction pathways and participate in intracellular signal transduction processes.

What is the regulatory mechanism of RPS2 expression?

The expression regulation mechanism of RPS2 involves multiple levels, including transcriptional level, post-transcriptional level, translation level and post-translational level. These regulatory mechanisms work together to ensure that RPS2 expression levels remain stable in cells.