Recombinant Human RPE protein(Ala2-Arg228), His-tagged

• Cat.No.: RPE-3234H
• Product Overview: Recombinant Human RPE (NP_954699.1) (Ala 2-Arg 228) was expressed in HEK293, with a polyhistidine tag at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N-His
• Protein length: Ala2-Arg228
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human RPE consists of 246 amino acids and has a calculated molecular mass of 27 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhRPE is approximately 30 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: RPE ribulose-5-phosphate-3-epimerase [ Homo sapiens ]
• Official Symbol: RPE
• Synonyms: RPE; ribulose-5-phosphate-3-epimerase; ribulose-phosphate 3-epimerase; RPE2-1; MGC2636
• Gene ID: 6120
• mRNA Refseq: NM_006916
• Protein Refseq: NP_008847
• MIM: 180480
• UniProt ID: Q96AT9