Recombinant Human AMY2B protein (Met1-Leu511), His-tagged

• Cat.No.: AMY2B-232H
• Product Overview: Recombinant human AMY2B (NP_066188.1) (Met1-Leu511) protein, fused with a polyhistidine tag at the C-terminus, was expressed in E. coli.

Specification

• CommonName: AMY2B
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Gln 16
• Form: Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
• Molecular Mass: The recombinant human AMY2B consists 507 amino acids and predicts a molecular mass of 57.3 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: AMY2B amylase, alpha 2B (pancreatic) [ Homo sapiens ]
• Official Symbol: AMY2B
• Synonyms: AMY2B; amylase, alpha 2B (pancreatic); AMY2, amylase, alpha 2B; pancreatic; alpha-amylase 2B; glycogenase; alpha-amylase carcinoid; carcinoid alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase 2B; AMY2
• Gene ID: 280
• mRNA Refseq: NM_020978
• Protein Refseq: NP_066188
• MIM: 104660
• UniProt ID: P19961

Reviews

08/30/2022

the manufacturer's supply management capabilities assure a seamless and continuous provision of the AMY2B protein.

05/06/2021

The AMY2B protein offered by the manufacturer exhibits exceptional quality and is precisely tailored to meet my experimental requirements.

10/14/2018

In addition to the remarkable quality of the protein, the manufacturer's support extends beyond the provision of the product itself.

08/23/2018

Its purity, stability, and functionality make it an ideal choice for my research endeavors.

01/24/2018

With their meticulous documentation and support, I am confident that my utilization of AMY2B protein in clinical trials will meet all necessary regulations and guidelines.

Q&As

Are there any genetic variations or polymorphisms associated with the AMY2B gene?

Yes, genetic variations and polymorphisms have been identified in the AMY2B gene. Similar to the AMY2A gene, the copy number of the AMY2B gene can vary among individuals. Some studies have suggested that individuals with higher AMY2B gene copy numbers may have a greater capacity for carbohydrate digestion and a lower risk of developing obesity and related metabolic disorders. However, further research is needed to fully understand the implications of these genetic variations on carbohydrate metabolism and health.

How does the AMY2B protein function in carbohydrate digestion?

The AMY2B protein functions by catalyzing the hydrolysis of alpha-1,4-glucosidic linkages in carbohydrates. It specifically targets starch and glycogen, which are found in various foods, including grains, potatoes, and legumes. AMY2B starts the breakdown process in the mouth, where it is secreted in saliva, and continues its action in the small intestine. It breaks down the complex carbohydrates into simpler sugars, such as glucose and maltose, which can be absorbed and metabolized by the body.

Are there any health implications associated with AMY2B gene duplication or deletion?

AMY2B gene duplication or deletion can potentially have health implications, although the exact consequences are still under investigation. Some studies have reported that individuals with higher AMY2B copy numbers may have a greater ability to digest and utilize starch, which could have implications for metabolic health and nutrient absorption.

Does the AMY2B protein have any other functions besides carbohydrate digestion?

While the primary function of the AMY2B protein is the digestion of carbohydrates, it may have additional roles in the body. Some studies have suggested that amylase enzymes, including AMY2B, might also have antibacterial properties and be involved in innate immunity. However, the extent and significance of these potential functions of the AMY2B protein are not fully understood and require further research to elucidate.

Are there any known pharmacological interventions that target AMY2B for therapeutic purposes?

Currently, there are no known pharmacological interventions specifically targeting AMY2B for therapeutic purposes. Most interventions related to carbohydrate digestion and metabolism primarily focus on digestive enzymes such as amylase or medications that affect glucose regulation, rather than directly targeting the AMY2B gene or its protein product. However, as research in this field continues to advance, it is possible that future therapeutic interventions may be developed to specifically target AMY2B and enhance carbohydrate metabolism.

What are the health implications of AMY2B gene variations or mutations?

The health implications of AMY2B gene variations or mutations are not well-understood. However, disruptions or alterations in the AMY2B gene could potentially affect the efficiency of carbohydrate digestion and metabolism. It is possible that individuals with certain AMY2B gene variations or mutations may have reduced amylase activity, leading to difficulties in carbohydrate digestion and potential metabolic consequences. Further research is needed to determine the specific health implications of AMY2B gene variations.

Are there any therapeutic applications or implications of studying the AMY2B protein?

Studying the AMY2B protein could have potential therapeutic applications and implications. A better understanding of AMY2B gene variation and its impact on carbohydrate digestion and metabolism could potentially lead to personalized dietary recommendations for individuals based on their genetic profile.

Is there any association between AMY2B gene variations and dietary adaptation?

Yes, some studies have suggested an association between AMY2B gene variations and dietary adaptation. Specifically, there is evidence to suggest that populations with a long history of high-starch diets, such as those who traditionally consume agricultural or processed foods, may have higher AMY2B copy numbers. It is believed that these genetic variations have evolved as an adaptation to the increased consumption of starch-rich foods, allowing for more efficient carbohydrate digestion and utilization.

Are there any known interactions between AMY2B gene variations and gut microbiota?

Research investigating the interactions between AMY2B gene variations and gut microbiota is emerging but still limited. Some studies have suggested that AMY2B gene copy number variations may influence the composition and function of the gut microbiota, which in turn can impact digestion and nutrient metabolism.

Can AMY2B gene variations be inherited?

Yes, AMY2B gene variations can be inherited. The AMY2B gene is located on chromosome 1, and variations in this gene can be passed down from parents to their offspring. Some studies have suggested that AMY2B copy number variations show heritability patterns, meaning they can be inherited within families. However, the inheritance patterns and mechanisms of AMY2B gene variations are still not fully understood, and more research is needed to uncover the specifics of their hereditary nature.

Does AMY2B gene variation affect an individual's response to a high-carbohydrate or low-carbohydrate diet?

The relationship between AMY2B gene variations and an individual's response to high-carbohydrate or low-carbohydrate diets is not fully understood. Some studies have examined the potential influence of AMY2B gene copy numbers on dietary responses and found that individuals with higher AMY2B copy numbers may have a more favorable response to high-carbohydrate diets in terms of insulin sensitivity and weight management.

Can changes in AMY2B expression or activity impact digestion and nutrient absorption?

Yes, changes in AMY2B expression or activity can impact digestion and nutrient absorption. Reduced expression or activity of AMY2B may lead to inadequate digestion of carbohydrates, resulting in malabsorption of nutrients and potential digestive discomfort such as bloating or gas.

Are there any diseases or conditions associated with AMY2B gene mutations?

At present, there is limited evidence to suggest specific diseases or conditions associated with AMY2B gene mutations. However, some studies have explored potential links between AMY2B gene variations and metabolic disorders such as obesity, diabetes, and metabolic syndrome.

How is AMY2B gene expression regulated?

The regulation of AMY2B gene expression is not yet fully understood. However, some studies have investigated potential regulators of AMY2B expression. It has been suggested that dietary factors, such as the consumption of high-starch diets, may induce AMY2B gene expression. Additionally, certain signaling pathways and transcription factors, such as CREB, Foxo1, and HNF1α, may be involved in regulating AMY2B gene expression. Further research is needed to elucidate the precise mechanisms and factors that regulate AMY2B gene expression.