Recombinant Human RPE65 Over-expression Lysate, Myc/DDK-tagged

• Cat.No.: SPRTN-01HCL
• Product Overview: Transient overexpression lysate of chromosome 1 open reading frame 124 (C1orf124), transcript variant 1 with a C-Myc/DDK tag was expressed in HEK293T.
  Components:
  1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 μg whole HEK293T cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Specification

• Description: The protein encoded by this gene may play a role in DNA repair during replication of damaged DNA. This protein recruits valosin containing protein (p97) to stalled DNA replication forks where it may prevent excessive translesional DNA synthesis and limit the number of DNA-damage induced mutations. It may also be involved in replication-related G2/M-checkpoint regulation. Deficiency of a similar protein in mouse causes chromosomal instability and progeroid phenotypes. Mutations in this gene have been associated with Ruijs-Aalfs syndrome (RJALS). Alternatively spliced transcript variants have been identified.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 55 kDa
• Stability: Lysate samples are stable for 12 months from the date of receipt when stored at -80 centigrade.
• Storage: Upon receiving, store the sample at -80 centigrade. Also after dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lysate samples can be diluted with 2×SDS Sample Buffer provided.
• Shipping: Dry ice.

Gene Information

• Gene Name: SPRTN SprT-like N-terminal domain [ Homo sapiens (human) ]
• Official Symbol: SPRTN
• Synonyms: SPRTN; SprT-like N-terminal domain; DVC1; PRO4323; spartan; C1orf124; DNA-dependent metalloprotease SPRTN; DNA damage protein targeting VCP; DNA damage-targeting VCP (p97) adaptor; zinc finger RAD18 domain-containing protein
• Gene ID: 83932
• mRNA Refseq: NM_032018
• Protein Refseq: NP_114407
• MIM: 616086
• UniProt ID: Q9H040