Recombinant Human RPS6KB1, His-tagged

• Cat.No.: RPS6KB1-31345TH
• Product Overview: Recombinant full-length human S6K (p70) contains an N-terminal His tag and was expressed by baculovirus in Sf9 insect cells.

Specification

• Description: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
• Conjugation: HIS
• Tissue specificity: Widely expressed.
• Biological activity: Note that this is the p70 form of S6K.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain.

Gene Information

• Gene Name: RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 [ Homo sapiens ]
• Official Symbol: RPS6KB1
• Synonyms: RPS6KB1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; ribosomal protein S6 kinase, 70kD, polypeptide 1 , STK14A; ribosomal protein S6 kinase beta-1; p70(S6K) alpha; PS6K; S6K1
• Gene ID: 6198
• mRNA Refseq: NM_003161
• Protein Refseq: NP_003152
• MIM: 608938
• Uniprot ID: P23443
• Chromosome Location: 17q23.1
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem
• Function: ATP binding; nucleotide binding; peptide binding; protein binding; protein kinase activity

Reviews

09/16/2021

Dependable protein purification, consistently high-quality results.

06/11/2021

Precise mass spectrometry analysis, essential for our proteomics work.

06/30/2019

Efficient peptide synthesis, crucial for our peptide research projects.

Q&As

What are the mechanisms by which RPS6KB1 modulates autophagy and cell survival?

RPS6KB1 regulates autophagy and cell survival, crucial for cellular health and response to stress.

How does RPS6KB1 (Ribosomal Protein S6 Kinase B1) regulate protein synthesis and cell growth?

RPS6KB1 controls protein synthesis and cell growth, playing a key role in cellular proliferation.

What is the impact of RPS6KB1 on cellular responses to stress and DNA damage?

RPS6KB1 impacts cellular stress responses, including the repair of DNA damage and maintenance of genomic stability.

How does RPS6KB1 contribute to the development and progression of cancer?

RPS6KB1 can contribute to cancer by promoting tumor cell growth and proliferation.

What role does RPS6KB1 play in the mTOR signaling pathway, particularly in response to nutrient availability?

RPS6KB1 is a critical component of the mTOR pathway, responding to nutrient signals to regulate cell metabolism and growth.

How do alterations in RPS6KB1 expression or activity affect aging and age-related diseases?

Changes in RPS6KB1 function can affect aging processes and the development of age-related diseases.

How does RPS6KB1 influence metabolic processes, especially in conditions like obesity and diabetes?

RPS6KB1 influences metabolic processes, affecting how cells respond to conditions like obesity and diabetes.