Recombinant Human RSPO1 protein, His & T7-tagged

• Cat.No.: RSPO1-1942H
• Product Overview: Recombinant Human RSPO1 aa. (Ser21~Ala135 (Accession # Q2MKA7)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.5kDa
• Protein length: Ser21~Ala135 (Accession # Q2MKA7)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 8.9

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens (human) ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; RSPO; CRISTIN3; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
• Gene ID: 284654
• mRNA Refseq: NM_001038633.3
• Protein Refseq: NP_001033722.1
• UniProt ID: Q2MKA7

Reviews

03/13/2022

Remarkable changes in cell behavior were attributed to the potent effects of the product.

01/16/2018

The product's respected reputation in the scientific community was a testament to its quality and effectiveness.

09/25/2016

Dependable protein-protein interaction outcomes were achieved due to the product's pure composition.

Q&As

What distinguishes RSPO1 from other RSPO molecules, particularly in terms of its potential therapeutic use in Regenerative Medicine?

RSPO1 stands out for its potential therapeutic use in Regenerative Medicine due to its mitogenic activity in stem cells.

What methods were employed to characterize the purified rhRSPO1, both structurally and biologically?

Characterization methods included Western blotting, mass spectrometry, and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming structural integrity and biological efficacy.

What tissue-specific processes are regulated by RSPOs, and what are some examples of these processes?

RSPOs regulate various tissue-specific processes, including bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells, intestinal stem cells, and cancer progression.

Describe the purification strategy used to obtain rhRSPO1, including the chromatographic steps involved.

The purification strategy for rhRSPO1 involved two sequential chromatographic steps: heparin affinity chromatography followed by molecular exclusion chromatography.

How does RSPO1 induce the Wnt pathway, and what are the downstream effects on cell proliferation, differentiation, and death?

RSPO1 induces the Wnt pathway by stabilizing the Wnt receptor complex, leading to enhanced Wnt signaling and influencing cellular processes.

How was recombinant human RSPO1 (rhRSPO1) generated, and which cell line was used for this purpose?

rhRSPO1 was generated using the HEK293 cell line, and the coding sequence was synthesized and subcloned into a mammalian cell expression vector.

What is the main function of R-Spondin proteins, including RSPO1, in cellular processes?

R-Spondin proteins, including RSPO1, play crucial roles in cell proliferation, differentiation, and death by inducing the Wnt pathway.