Recombinant Human S-adenosyl-L-homocysteine hydrolase

• Cat.No.: AHCY-2H

Specification

• Description: S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Species: Human
• Form: Liquid
• Purity: Greater than 90% as determined by Analysis by SDS-PAGE.
• Usage: This product as supplied is intended forresearch use only, not for use in human, therapeutic or diagnostic applications.
• Storage Buffer: 20 mM Tris pH 7.5, 20% glycerol, 1‰NaN3

Gene Information

• Gene Name: AHCY adenosylhomocysteinase [ Homo sapiens ]
• Official Symbol: AHCY
• Synonyms: AHCY; adenosylhomocysteinase; S adenosylhomocysteine hydrolase; SAHH; adoHcyase; S-adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase
• Gene ID: 191
• mRNA Refseq: NM_000687
• Protein Refseq: NP_000678
• MIM: 180960
• UniProt ID: P23526
• Chromosome Location: 20q11.22
• Pathway: Biological oxidations, organism-specific biosystem; Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Methionine degradation, organism-specific biosystem
• Function: adenosylhomocysteinase activity; hydrolase activity

Reviews

03/16/2022

My experiments have never been smoother since I started using this protein.

10/03/2021

In our scientific pursuits, this lab protein has surpassed expectations, demonstrating exceptional quality and ease of use.

01/04/2020

I highly recommend this protein to fellow researchers. It's a true gem!

07/11/2019

We wholeheartedly endorse the lab protein product for its outstanding performance and its significant contribution to our research outcomes.

12/31/2018

This protein is like a reliable teammate in the lab – always delivering excellent results.

Q&As

What is the significance of AHCY in maintaining cellular methylation homeostasis?

By degrading SAH, AHCY ensures the availability of S-adenosylmethionine (SAM), a universal methyl donor essential for DNA, RNA, and protein methylation reactions.

Explain the involvement of AHCY in the pathogenesis of liver diseases, such as non-alcoholic fatty liver disease (NAFLD).

Dysregulation of AHCY and altered methylation patterns have been linked to the development and progression of NAFLD and other liver pathologies.

How does AHCY catalyze the hydrolysis of S-adenosylhomocysteine?

AHCY employs a zinc-dependent mechanism to cleave the N-ribosidic bond of SAH, generating adenosine and homocysteine as products.

Describe the AHCY-related therapeutic approaches for diseases linked to aberrant methylation patterns.

Targeting AHCY activity or SAM availability holds promise as a potential therapeutic strategy for conditions influenced by disrupted methylation.

How do genetic variations in AHCY contribute to disease susceptibility?

Single nucleotide polymorphisms (SNPs) in the AHCY gene can affect enzyme activity and SAH levels, potentially influencing disease risk, including cardiovascular disorders and neurodegenerative diseases.

What are the metabolic fates of homocysteine generated by AHCY activity?

Homocysteine can be remethylated to methionine through the folate-dependent pathway or transsulfurated to cysteine via the transsulfuration pathway.

Discuss the crosstalk between AHCY and folate metabolism in one-carbon metabolism.

AHCY activity is intimately connected with folate-mediated one-carbon metabolism, affecting the availability of methyl groups for various methylation reactions.

How is AHCY expression regulated in response to cellular demands?

AHCY expression can be modulated by transcriptional factors and epigenetic modifications, responding to changes in cellular methylation needs.

How does AHCY interact with other cellular pathways, such as the polyamine biosynthesis pathway?

AHCY indirectly influences polyamine biosynthesis by providing the methyl groups required for polyamine synthesis.

Describe the structural features of AHCY that contribute to its catalytic efficiency.

AHCY possesses a TIM-barrel fold with an active site that houses the zinc ion critical for its hydrolytic activity.