Recombinant Human S100B Protein, His-tagged

• Cat.No.: S100B-64H
• Product Overview: Recombinant Human S100 Calcium Binding Protein B is produced by our E.coli expression system and the target gene encoding Met1-Glu92 is expressed with a 6His tag at the N-terminus.

Specification

• Description: S100-B, is an acidic protein with a molecular weight of 21 kDa belonging to the S100 family. S100-B contains two EF-hand-type calcium-binding motifs separated by a hinge region with a hydrophobic cleft. S100-B plays an important role in neurodevelopment, differentiation, and brain construction. S100-B has neuroprotective effects, but at high concentrations S100-B is neurotoxic. Extracellular concentration of S100-B increases following brain damage, which easily penetrates into cerebrospinal fluid in brain damage and then into the blood. S100-B is expressed and produced by astrocytes in vertebrate brains and in the CNS, and the astrocytes are the major cells producing S100-B protein in gray matter, as well as oligodendrocytes are the predominant S100-B in protein producing cells in white matter. The major advantage of using S100-B is that elevations in serum or CSF levels provide a sensitive measure for determining CNS injury at the molecular level before gross changes develop, enabling timely delivery of crucial medical intervention before irreversible damage occurs. In addition, S100-B, which is also present in human melanocytes, is a reliable marker for melanoma malignancy both in bioptic tissue and in serum.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 um filtered solution of 20mM PBS,150mM NaCl, pH7.4.
• Protein Length: Met1-Glu92
• AA Sequence: MNHKVHHHHHHMSELEKAMVALIDV FHQYSGREGDKHKLKKSELKELINN ELSHFLEEIKEQEVVDKVM ETLDNDGDGECDF QEFMAFVAMVTTACHEFFEHEC
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20centigrade for 3 months.
• Quality Statement: Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: S100B S100 calcium binding protein B [ Homo sapiens (human) ]
• Official Symbol: S100B
• Synonyms: Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100b; S100 beta; S100 calcium binding protein B; NEF; S100; S100-B; S100beta
• Gene ID: 6285
• mRNA Refseq: NM_006272.3
• Protein Refseq: NP_006263.1
• MIM: 176990
• UniProt ID: P04271