Recombinant Human SETDB1 Protein (1-397 aa), GST-tagged

• Cat.No.: SETDB1-2120H
• Product Overview: Recombinant Human SETDB1 Protein (1-397 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Full Length of Isoform 2.

Specification

• Description: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing . The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 71.7 kDa
• Protein length: 1-397 aa
• AA Sequence: MSSLPGCIGLDAATATVESEEIAEL QQAVVEELGISMEELRHFIDEELEK MDCVQQRKKQLAELETWVIQKESEV AHVDQLFDDASRAVTNCESLVKDFY SKLGLQYRDSSSEDESSRPTEIIEI PDEDDDVLSIDSGDAGSRTPKDQKL REAMAALRKSAQDVQKFMDAVNKKS SSQDLHKGTLSQMSGELSKDGDLIV SMRILGKKRTKTWHKGTLIAIQTVG PGKKYKVKFDNKGKSLLSGNHIAYD YHPPADKLYVGSRVVAKYKDGNQVW LYAGIVAETPNVKNKLRFLIFFDDG YASYVTQSELYPICRPLKKTWEDIE DISCRDFIEEYVTAYPNRPMVLLKS GQLIKTEWEGTWWKSRVEEVDGSLV RILFLVLFFSTILEAEVGGGGT
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: SETDB1 SET domain, bifurcated 1 [ Homo sapiens ]
• Official Symbol: SETDB1
• Synonyms: SETDB1; ESET; KG1T; KIAA0067; KMT1E; H3-K9-HMTase 4; H3-K9-HMTase4
• Gene ID: 9869
• mRNA Refseq: NM_001145415
• Protein Refseq: NP_001138887
• MIM: 604396
• UniProt ID: Q15047