| Description : |
This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
100.7 kDa |
| AA Sequence : |
MATEHPEPPKAELQLPPPPPPGHYG AWAAQELQAKLAEIGAPIQGNREEL VERLQSYTRQTGIVLNRPVLRGEDG DKAAPPPMSAQLPGIPMPPPPLGLP PLQPPPPPPPPPPGLGLGFPMAHPP NLGPPPPLRVGEPVALSEEERLKLA QQQAALLMQQEERAKQQGDHSLKEH ELLEQQKRAAVLLEQERQQEIAKMG TPVPRPPQDMGQIGVRTPLGPRVAA PVGPVGPTPTVLPMGAPVPRPRGPP PPPGDENREMDDPSVGPKIPQALEK ILQLKESRQEEMNSQQEEEEMETDA RSSLGQSASETEEDTVSVSKKEKNR KRRNRKKKKKPQRVRGVSSESSGDR EKDSTRSRGSDSPAADVEIEYVTEE PEIYEPNFIFFKRIFEAFKLTDDVK KEKEKEPEKLDKLENSAAPKKKGFE EEHKDSDDDSSDDEQEKKPEAPKLS KKKLRRMNRFTVAELKQLVARPDVV EMHDVTAQDPKLLVHLKATRNSVPV PRHWCFKRKYLQGKRGIEKPPFELP DFIKRTGIQEMREALQEKEEQKTMK SKMREKVRPKMGKIDIDYQKLHDAF FKWQTKPKLTIHGDLYYEGKEFETR LKEKKPGDLSDELRISLGMPVGPNA HKVPPPWLIAMQRYGPPPSYPNLKI PGLNSPIPESCSFGYHAGGWGKPPV DETGKPLYGDVFGTNAAEFQTKTEE EEIDRTPWGELEPSDEESSEEEEEE ESDEDKPDETGFITPADSGLITPGG FSSVPAGMETPELIELRKKKIEEAM DGSETPQLFTVLPEKRTATVGGAMM GSTHIYDMSTVMSRKGPAPELQGVE VALAPEELELDPMAMTQKYEEHVRE QQAQVEKEDFSDMVAEHAAKQKQKK RKAQPQDSRGGSKKYKEFKFTRTRP LEQKLISEEDLAANDILDYKDDDDK V |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
