Recombinant Human SIAE protein(Met1-Lys523), His-tagged

• Cat.No.: SIAE-7357H
• Product Overview: Recombinant Human SIAE (Met 1-Lys523) (Q9HAT2-1) was expressed in Insect Cells, with a C-terminal polyhistidine tag.

Specification

• Source: Insect Cells
• Species: Human
• Tag: C-His
• Protein length: Met1-Lys523
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human SIAE consists of 511 amino acids and predicts a molecular mass of 57.4 KDa. The apparent molecular mass of the protein is approximately 61 Kda in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SIAE sialic acid acetylesterase [ Homo sapiens ]
• Official Symbol: SIAE
• Synonyms: SIAE; sialic acid acetylesterase; YSG2, Ysg2 homolog (mouse); sialate O-acetylesterase; CSE C; LSE; MGC87009; sialic acid specific acetylesterase II; H-Lse; sialic acid-specific acetylesterase II; cytosolic sialic acid 9-O-acetylesterase homolog; AIS6; CSEC; YSG2; CSE-C
• Gene ID: 54414
• mRNA Refseq: NM_001199922
• Protein Refseq: NP_001186851
• MIM: 610079
• UniProt ID: Q9HAT2