Recombinant Human SIRPA protein(Met1-Arg370), His-Avi-tagged, Biotinylated
Cat.No. : | SIRPA-1170H |
Product Overview : | Biotinylated Recombinant Human SIRPA (NP_542970.1) (Met1-Arg370) was expressed in HEK293 with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | C-His-Avi |
Protein length : | Met1-Arg370 |
Conjugation/Label : | Biotin |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized CD47-Fc at 10 μg/mL (100 μL/well) can bind SIRPA-AvihL, the EC50 of SIRPA-AvihL is 200-500 ng/mL. |
Molecular Mass : | The recombinant human SIRPA consists of 366 amino acids and predicts a molecular mass of 40.6 kDa. |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 90 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | SIRPA signal-regulatory protein alpha [ Homo sapiens ] |
Official Symbol : | SIRPA |
Synonyms : | SIRPA; signal-regulatory protein alpha; protein tyrosine phosphatase, non receptor type substrate 1 , PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; MYD-1; CD172A; PTPNS1; |
Gene ID : | 140885 |
mRNA Refseq : | NM_001040022 |
Protein Refseq : | NP_001035111 |
MIM : | 602461 |
UniProt ID : | P78324 |
Products Types
◆ Recombinant Protein | ||
SIRPA-723H | Recombinant Human SIRPA Protein, Fc-tagged | +Inquiry |
SIRPA-019H | Recombinant Human SIRPA Protein, C-His-tagged | +Inquiry |
SIRPA-1158C | Recombinant Cynomolgus SIRPA protein(Met1-Tyr372), His-tagged | +Inquiry |
SIRPA-2B | Recombinant Bovine SIRPA Protein, His (Fc)-Avi-tagged | +Inquiry |
SIRPA-1251M | Acitve Recombinant Mouse SIRPA protein(Met1-Asn373), His-tagged | +Inquiry |
◆ Lysates | ||
SIRPA-2298HCL | Recombinant Human SIRPA cell lysate | +Inquiry |
SIRPA-1650MCL | Recombinant Mouse SIRPA cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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