Recombinant Human SPRED2 protein, His & T7-tagged

• Cat.No.: SPRED2-1802H
• Product Overview: Recombinant Human SPRED2 aa. (Met1~Ala418 (Accession # Q7Z698)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 51.2kDa
• Protein length: Met1~Ala418 (Accession # Q7Z698)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 6.3

Gene Information

• Gene Name: SPRED2 sprouty related EVH1 domain containing 2 [ Homo sapiens (human) ]
• Official Symbol: SPRED2
• Synonyms: SPRED2; sprouty related EVH1 domain containing 2; Spred-2; sprouty-related, EVH1 domain-containing protein 2; sprouty protein with EVH-1 domain 2, related sequence
• Gene ID: 200734
• mRNA Refseq: NM_001128210.1
• Protein Refseq: NP_001121682.1
• UniProt ID: Q7Z698