Recombinant Human SUCLA2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SUCLA2-4800H
• Product Overview: SUCLA2 MS Standard C13 and N15-labeled recombinant protein (NP_003841) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 50.3 kDa
• AA Sequence: MAASMFYGRLVAVATLRNHRPRTAQ RAAAQVLGSSGLFNNHGLQVQQQQQ RNLSLHEYMSMELLQEAGVSVPKGY VAKSPDEAYAIAKKLGSKDVVIKAQ VLAGGRGKGTFESGLKGGVKIVFSP EEAKAVSSQMIGKKLFTKQTGEKGR ICNQVLVCERKYPRREYYFAITMER SFQGPVLIGSSHGGVNIEDVAAESP EAIIKEPIDIEEGIKKEQALQLAQK MGFPPNIVESAAENMVKLYSLFLKY DATMIEINPMVEDSDGAVLCMDAKI NFDSNSAYRQKKIFDLQDWTQEDER DKDAAKANLNYIGLDGNIGCLVNGA GLAMATMDIIKLHGGTPANFLDVGG GATVHQVTEAFKLITSDKKVLAILV NIFGGIMRCDVIAQGIVMAVKDLEI KIPVVVRLQGTRVDDAKALIADSGL KILACDDLDEAARMVVKLSEIVTLA KQAHVDVKFQLPITRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SUCLA2 succinate-CoA ligase ADP-forming subunit beta [ Homo sapiens (human) ]
• Official Symbol: SUCLA2
• Synonyms: SUCLA2; succinate-CoA ligase ADP-forming subunit beta; A-SCS; A-BETA; MTDPS5; LINC00444; SCS-betaA; succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial; ATP-specific succinyl-CoA synthetase subunit beta; ATP-specific succinyl-CoA synthetase, beta subunit; long intergenic non-protein coding RNA 444; mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta; renal carcinoma antigen NY-REN-39; succinate-CoA ligase ADP-forming beta subunit; succinate-CoA ligase beta subunit; succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial; succinyl-CoA synthetase beta-A chain; EC 6.2.1.5
• Gene ID: 8803
• mRNA Refseq: NM_003850
• Protein Refseq: NP_003841
• MIM: 603921
• UniProt ID: Q9P2R7