Recombinant Human SUFU, His-tagged

• Cat.No.: SUFU-31479TH
• Product Overview: Recombinant full-length Human Suppressor of Fused with a N terminal His tag; 504 amino acids with a predicted MWt 56.1 kDa including tag.

Specification

• Description: The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.
• Protein length: 484 amino acids
• Conjugation: HIS
• Molecular Weight: 56.100kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Ubiquitous in adult tissues. Detected in osteoblasts of the perichondrium in the developing limb of 12-week old embryos. Isoform 1 is detected in fetal brain, lung, kidney and testis. Isoform 2 is detected in fetal testis, and at much lower levels in feta
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 0.03% DTT, 10% Glycerol, 0.79% Sodium chloride
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MAELRPSGAP GPTAPPAPGP TAPPAFASLF PPGLHAIYGE CRRLYPDQPN PLQVTAIVKY WLGGPDPLDY VSMYRNVGSP SANIPEHWHY ISFGLSDLYG DNRVHEFTGT DGPSGFGFEL TFRLKRETGE SAPPTWPAEL MQGLARYVFQ SENTFCSGDH VSWHSPLDNS ESRIQHMLLT EDPQMQPVQT PFGVVTFLQI VGVCTEELHS AQQWNGQGIL ELLRTVPIAG GPWLITDMRR GETIFEIDPH LQERVDKGIE TDGSNLSGVS AKCAWDDLSR PPEDDEDSRS ICIGTQPRRL SGKDTEQIRE TLRRGLEINS KPVLPPINPQ RQNGLAHDRA PSRKDSLESD SSTAIIPHEL IRTRQLESVH LKFNQESGAL IPLCLRGRLL HGRHFTYKSI TGDMAITFVS TGVEGAFATE EHPYAAHGPW LQILLTEEFV EKMLEDLEDL TSPEEFKLPK EYSWPEKKLK VSILPDVVFD SPLH
• Sequence Similarities: Belongs to the SUFU family.

Gene Information

• Gene Name: SUFU suppressor of fused homolog (Drosophila) [ Homo sapiens ]
• Official Symbol: SUFU
• Synonyms: SUFU; suppressor of fused homolog (Drosophila); suppressor of fused homolog; PRO1280; SUFUH; SUFUXL
• Gene ID: 51684
• mRNA Refseq: NM_001178133
• Protein Refseq: NP_001171604
• MIM: 607035
• Uniprot ID: Q9UMX1
• Chromosome Location: 10q24.32
• Pathway: Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Hedgehog Signaling Pathway, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem; Hedgehog signaling pathway, organism-specific biosystem
• Function: beta-catenin binding; protein binding; protein kinase binding; signal transducer activity; transcription corepressor activity