Recombinant Human TET3 Protein (824-1795), N-6xHis and Flag-tagged

• Cat.No.: TET3-13H
• Product Overview: Recombinant TET3 (824-1795) protein that includes amino acids 824-1795 of human TET3 protein (accession number NP_001274420.1) was expressed in Sf9 cells and contains an N-terminal 6xHis Tag and FLAG-Tag with a molecular weight of 111.3 kDa. The recombinant protein is >30% pure by SDS-PAGE.

Specification

• Description: Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process.
• Source: Baculovirus
• Species: Human
• Tag: N-6xHis and Flag
• Molecular Mass: 111.3 kDa
• Protein Length: 824-1795
• Applications: Recombinant TET3 (824-1795) protein is suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling.
• Usage: 250 nM of 30bp DNA oligos (sequence: 5'-CAGTAACTGTGGTC(mC)GGTAACTGACTTGCA-3', contains a 5mCpG site) was incubated with 100 nM recombinant TET3 (824-1795) protein in buffer containing 50 mM HEPES, pH 8.0, 100 μM Fe(NH4)2(SO4)2, 2 mM ascorbate, 1 mM alpha-ketoglutarate, 1 mM ATP, 1 mM TCEP at 37 centigrade for 1 hr. Activity was detected by Dot-blot.
• Notes: This product is for research use only and is not for use in diagnostic procedures.
• Stability: This product is guaranteed for 6 months from date of arrival.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.1 μg/μL

Gene Information

• Gene Name: TET3 tet methylcytosine dioxygenase 3 [ Homo sapiens (human) ]
• Official Symbol: TET3
• Synonyms: TET3; tet methylcytosine dioxygenase 3; BEFAHRS; hCG_40738; methylcytosine dioxygenase TET3; probable methylcytosine dioxygenase TET3; putative methylcytosine dioxygenase; ten-eleven translocation 3; tet oncogene family member 3; EC 1.14.11.n2
• Gene ID: 200424
• mRNA Refseq: NM_001287491
• Protein Refseq: NP_001274420
• MIM: 613555
• UniProt ID: O43151