Recombinant Human TFB2M Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TFB2M-696H
• Product Overview: TFB2M MS Standard C13 and N15-labeled recombinant protein (NP_071761) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: S-adenosyl-L-methionine-dependent rRNA methyltransferase which may methylate two specific adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 12S mitochondrial rRNA (Probable). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Stimulates transcription independently of the methyltransferase activity.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 45.3 kDa
• AA Sequence: MWIPVVGLPRRLRLSALAGAGRFCI LGSEAATRKHLPARNHCGLSDSSPQ LWPEPDFRNPPRKASKASLDFKRYV TDRRLAETLAQIYLGKPSRPPHLLL ECNPGPGILTQALLEAGAKVVALES DKTFIPHLESLGKNLDGKLRVIHCD FFKLDPRSGGVIKPPAMSSRGLFKN LGIEAVPWTADIPLKVVGMFPSRGE KRALWKLAYDLYSCTSIYKFGRIEV NMFIGEKEFQKLMADPGNPDLYHVL SVIWQLACEIKVLHMEPWSSFDIYT RKGPLENPKRRELLDQLQQKLYLIQ MIPRQNLFTKNLTPMNYNIFFHLLK HCFGRRSATVIDHLRSLTPLDARDI LMQIGKQEDEKVVNMHPQDFKTLFE TIERSKDCAYKWLYDETLEDRTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TFB2M transcription factor B2, mitochondrial [ Homo sapiens (human) ]
• Official Symbol: TFB2M
• Synonyms: TFB2M; transcription factor B2, mitochondrial; dimethyladenosine transferase 2, mitochondrial; FLJ22661; FLJ23182; Hkp1; hTFB2M; h-mtTFB; h-mtTFB2; HCV NS5A-transactivated protein 5; mitochondrial 12S rRNA dimethylase 2; mitochondrial transcription factor B2; hepatitis C virus NS5A-transactivated protein 5; S-adenosylmethionine-6-N, N-adenosyl(rRNA) dimethyltransferase 2; mtTFB2
• Gene ID: 64216
• mRNA Refseq: NM_022366
• Protein Refseq: NP_071761
• MIM: 607055
• UniProt ID: Q9H5Q4