Recombinant Human TFPI2 protein(Met1-Lys213), His-tagged

• Cat.No.: TFPI2-876H
• Product Overview: Recombinant Human TFPI2 (NP_006519.1)(Met 1-Lys 213) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Lys213
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is < 2 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin, 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7. 5.
• Molecular Mass: The secreted recombinant humanTFPI2 comprises 202 amino acids and predicts a molecular mass of 23.2 kDa. It migrates as an approximately 32-34 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TFPI2 tissue factor pathway inhibitor 2 [ Homo sapiens ]
• Official Symbol: TFPI2
• Synonyms: TFPI2; tissue factor pathway inhibitor 2; PP5; REF1; TFPI 2; placental protein 5; TFPI-2; FLJ21164
• Gene ID: 7980
• mRNA Refseq: NM_006528
• Protein Refseq: NP_006519
• MIM: 600033
• UniProt ID: P48307