Recombinant Human TGS1 Protein (713-853 aa), His-SUMO-tagged

• Cat.No.: TGS1-1092H
• Product Overview: Recombinant Human TGS1 Protein (713-853 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial.

Specification

• Description: Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m7G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 31.6 kDa
• Protein length: 713-853 aa
• AA Sequence: MRVIAIDIDPVKIALARNNAEVYGI ADKIEFICGDFLLLASFLKADVVFL SPPWGGPDYATAETFDIRTMMSPDG FEIFRLSKKITNNIVYFLPRNADID QVASLAGPGGQVEIEQNFLNNKLKT ITAYFGDLIRRPASET
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: TGS1 trimethylguanosine synthase 1 [ Homo sapiens ]
• Official Symbol: TGS1
• Synonyms: TGS1; PIMT; SEREX-defined; PIPMT; NCOA6IP; FLJ22995; DKFZp762A163
• Gene ID: 96764
• mRNA Refseq: NM_024831
• Protein Refseq: NP_079107
• MIM: 606461
• UniProt ID: Q96RS0