| Description : |
This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
195.3 kDa |
| AA Sequence : |
MSARAAAAKSTAMEETAIWEQHTVT LHRAPGFGFGIAISGGRDNPHFQSG ETSIVISDVLKGGPAEGQLQENDRV AMVNGVSMDNVEHAFAVQQLRKSGK NAKITIRRKKKVQIPVSRPDPEPVS DNEEDSYDEEIHDPRSGRSGVVNRR SEKIWPRDRSASRERSLSPRSDRRS VASSQPAKPTKVTLVKSRKNEEYGL RLASHIFVKEISQDSLAARDGNIQE GDVVLKINGTVTENMSLTDAKTLIE RSKGKLKMVVQRDERATLLNVPDLS DSIHSANASERDDISEIQSLASDHS GRSHDRPPRRSRSRSPDQRSEPSDH SRHSPQQPSNGSLRSRDEERISKPG AVSTPVKHADDHTPKTVEEVTVERN EKQTPSLPEPKPVYAQVGQPDVDLP VSPSDGVLPNSTHEDGILRPSMKLV KFRKGDSVGLRLAGGNDVGIFVAGV LEDSPAAKEGLEEGDQILRVNNVDF TNIIREEAVLFLLDLPKGEEVTILA QKKKDVYRRIVESDVGDSFYIRTHF EYEKESPYGLSFNKGEVFRVVDTLY NGKLGSWLAIRIGKNHKEVERGIIP NKNRAEQLASVQYTLPKTAGGDRAD FWRFRGLRSSKRNLRKSREDLSAQP VQTKFPAYERVVLREAGFLRPVTIF GPIADVAREKLAREEPDIYQIAKSE PRDAGTDQRSSGIIRLHTIKQIIDQ DKHALLDVTPNAVDRLNYAQWYPIV VFLNPDSKQGVKTMRMRLCPESRKS ARKLYERSHKLRKNNHHLFTTTINL NSMNDGWYGALKEAIQQQQNQLVWV SEGKADGATSDDLDLHDDRLSYLSA PGSEYSMYSTDSRHTSDYEDTDTEG GAYTDQELDETLNDEVGTPPESAIT RSSEPVREDSSGMHHENQTYPPYSP QAQPQPIHRIDSPGFKPASQQKAEA SSPVPYLSPETNPASSTSAVNHNVN LTNVRLEEPTPAPSTSYSPQADSLR TPSTEAAHIMLRDQEPSLSSHVDPT KVYRKDPYPEEMMRQNHVLKQPAVS HPGHRPDKEPNLTYEPQLPYVEKQA SRDLEQPTYRYESSSYTDQFSRNYE HRLRYEDRVPMYEEQWSYYDDKQPY PSRPPFDNQHSQDLDSRQHPEESSE RGYFPRFEEPAPLSYDSRPRYEQAP RASALRHEEQPAPGYDTHGRLRPEA QPHPSAGPKPAESKQYFEQYSRSYE QVPPQGFTSRAGHFEPLHGAAAVPP LIPSSQHKPEALPSNTKPLPPPPTQ TEEEEDPAMKPQSVLTRVKMFENKR SASLETKKDVNDTGSFKPPEVASKP SGAPIIGPKPTSQNQFSEHDKTLYR IPEPQKPQLKPPEDIVRSNHYDPEE DEEYYRKQLSYFDRRSFENKPPAHI AASHLSEPAKPAHSQNQSNFSSYSS KGKPPEADGVDRSFGEKRYEPIQAT PPPPPLPSQYAQPSQPVTSASLHIH SKGAHGEGNSVSLDFQNSLVSKPDP PPSQNKPATFRPPNREDTAQAAFYP QKSFPDKAPVNGTEQTQKTVTPAYN RFTPKPYTSSARPFERKFESPKFNH NLLPSETAHKPDLSSKTPTSPKTLV KSHSLAQPPEFDSGVETFSIHAEKP KYQINNISTVPKAIPVSPSAVEEDE DEDGHTVVATARGIFNSNGGVLSSI ETGVSIIIPQGAIPEGVEQEIYFKV CRDNSILPPLDKEKGETLLSPLVMC GPHGLKFLKPVELRLPHCDPKTWQN KCLPGDPNYLVGANCVSVLIDHFTR TRPLEQKLISEEDLAANDILDYKDD DDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
