| Description : |
Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses. TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm. TLR2 is expressed on the surface of monocytes and macrophages and can heterodimerize with TLR1 or TLR6, enabling responses to a variety of pathogen-associated molecular patterns including lipopetides and peptidoglycan. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Molecular Mass : |
~68 kDa |
| AA Sequence : |
ESSNQASLSCDRNGICKGSSGSLNS IPSGLTEAVKSLDLSNNRITYISNS DLQRCVNLQALVLTSNGINTIEEDS FSSLGSLEHLDLSYNYLSNLSSSWF KPLSSLTFLNLLGNPYKTLGETSLF SHLTKLQILRVGNMDTFTKIQRKDF AGLTFLEELEIDASDLQSYEPKSLK SIQNVSHLILHMKQHILLLEIFVDV TSSVECLELRDTDLDTFHFSELSTG ETNSLIKKFTFRNVKITDESLFQVM KLLNQISGLLELEFDDCTLNGVGNF RASDNDRVIDPGKVETLTIRRLHIP RFYLFYDLSTLYSLTERVKRITVEN SKVFLVPCLLSQHLKSLEYLDLSEN LMVEEYLKNSACEDAWPSLQTLILR QNHLASLEKTGETLLTLKNLTNIDI SKNSFHSMPETCQWPEKMKYLNLSS TRIHSVTGCIPKTLEILDVSNNNLN LFSLNLPQLKELYISRNKLMTLPDA SLLPMLLVLKISRNAITTFSKEQLD SFHTLKTLEAGGNNFICSCEFLSFT QEQQALAKVLIDWPANYLCDSPSHV RGQQVQDVRLSVSECHRT |
| Purity : |
Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE). |
| Notes : |
For research use only, not for use in diagnostic procedure. |
| Storage : |
Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles. |
| Storage Buffer : |
PBS, 4M Urea, pH7.4 |
