Recombinant Human TNFSF15 Trimer protein, N-His-Flag-tagged, Biotinylated

• Cat.No.: TNFSF15-150HB
• Product Overview: Biotinylated Recombinant Human TNFSF15 Trimer protein(O95150-1)(Asp91-Leu251) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N-Flag-His
• Protein length: Asp91-Leu251
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse DR3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 (Trimer), His Tag with the EC50 of 0.16μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFSF15 tumor necrosis factor (ligand) superfamily, member 15 [ Homo sapiens ]
• Official Symbol: TNFSF15
• Synonyms: TNFSF15; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand superfamily member 15; MGC129934; MGC129935; TL1; TL1A; TNF ligand related molecule 1; TNF superfamily ligand TL1A; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor 192A; VEGI; VEGI192A; TNF ligand-related molecule 1; vascular endothelial growth inhibitor-192A
• Gene ID: 9966
• mRNA Refseq: NM_001204344
• Protein Refseq: NP_001191273
• MIM: 604052
• UniProt ID: O95150