Recombinant Human TPBG Protein, His\Avi-tagged

• Cat.No.: TPBG-198H
• Product Overview: Biotinylated Recombinant Human Trophoblast Glycoprotein is produced by our Mammalian expression system and the target gene encoding Ser32-Ser355 is expressed with a Avi, 6His tag at the C-terminus.

Specification

• Description: Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
• Source: Human Cells
• Species: Human
• Tag: His\Avi
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Molecular Mass: Predicted Molecular Weight: 38kDa
  Apparent Molecular Weight: 60-90kDa, reducing conditions.
• AA Sequence: Ser32-Ser355
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: TPBG trophoblast glycoprotein [ Homo sapiens (human) ]
• Official Symbol: TPBG
• Synonyms: 5T4; 5T4AG; M6P1; WAIF1
• Gene ID: 7162
• mRNA Refseq: NM_001166392.2
• Protein Refseq: NP_001159864.1
• MIM: 190920
• UniProt ID: Q13641