Recombinant Human TRIB3, GST-tagged

• Cat.No.: TRIB3-25H
• Product Overview: Recombinant full-length human TRIB3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: TRIB3 or tribbles homolog 3 protein encode one of three members of the Tribbles family that share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. TRIB3 is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. TRIB3 can negatively regulate the cell survival serine-threonine kinase AKT1. TRIB3 modulation by H. pylori and/or its products may be an important mechanism of H. pylori-associated pathogenesis. TRIB3 is a p65-interacting negative regulator of NF-kappa-B-dependent transcription. TRIB3 is highly expressed in pancreas, peripheral blood leukocytes, and bone marrow.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~69 kDa
• Applications: Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: TRIB3 tribbles homolog 3 (Drosophila) [ Homo sapiens ]
• Official Symbol: TRIB3
• Synonyms: TRIB3; tribbles homolog 3 (Drosophila); C20orf97, chromosome 20 open reading frame 97; tribbles homolog 3; dJ1103G7.3; TRB3; TRB-3; p65-interacting inhibitor of NF-kappaB; p65-interacting inhibitor of NF-kappa-B; neuronal cell death inducible putative kinase; neuronal cell death-inducible putative kinase; NIPK; SINK; SKIP3; C20orf97
• Gene ID: 57761
• mRNA Refseq: NM_021158
• Protein Refseq: NP_066981
• MIM: 607898
• UniProt ID: Q96RU7
• Chromosome Location: 20p13-p12.2
• Pathway: Activation of PKB, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Adipogenesis, organism-specific biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem; Costimulation by the CD28 family, organism-specific biosystem; Disease, organism-specific biosystem
• Function: ATP binding; enzyme binding; NOT kinase activity; protein binding; protein kinase binding; protein kinase binding; protein kinase inhibitor activity; transcription corepressor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity