Recombinant Human TWNK Protein (32-684 aa), His-tagged

• Cat.No.: TWNK-1698H
• Product Overview: Recombinant Human TWNK Protein (32-684 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Full Length of Mature Protein.

Specification

• Description: Involved in mitochondrial DNA (mtDNA) metabolism. Could function as an adenine nucleotide-dependent DNA helicase. Function inferred to be critical for lifetime maintenance of mtDNA integrity. In vitro, forms in combination with POLG, a processive replication machinery, which can use double-stranded DNA (dsDNA) as tplate to synthesize single-stranded DNA (ssDNA) molecules. May be a key regulator of mtDNA copy number in mammals.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 75.5 kDa
• Protein length: 32-684 aa
• AA Sequence: APGPPRRRYRKETLQALDMPVLPVT ATEIRQYLRGHGIPFQDGHSCLRAL SPFAESSQLKGQTGVTTSFSLFIDK TTGHFLCMTSLAEGSWEDFQASVEG RGDGAREGFLLSKAPEFEDSEEVRR IWNRAIPLWELPDQEEVQLADTMFG LTKVTDDTLKRFSVRYLRPARSLVF PWFSPGGSGLRGLKLLEAKCQGDGV SYEETTIPRPSAYHNLFGLPLISRR DAEVVLTSRELDSLALNQSTGLPTL TLPRGTTCLPPALLPYLEQFRRIVF WLGDDLRSWEAAKLFARKLNPKRCF LVRPGDQQPRPLEALNGGFNLSRIL RTALPAWHKSIVSFRQLREEVLGEL SNVEQAAGLRWSRFPDLNRILKGHR KGELTVFTGPTGSGKTTFISEYALD LCSQGVNTLWGSFEISNVRLARVML TQFAEGRLEDQLDKYDHWADRFEDL PLYFMTFHGQQSIRTVIDTMQHAVY VYDICHVIIDNLQFMMGHEQLSTDR IAAQDYIIGVFRKFATDNNCHVTLV IHPRKEDDDKELQTASIFGSAKASQ EADNVLILQDRKLVTGPGKRYLQVS KNRFDGDVGVFPLEFNKNSLTFSIP PKNKARLKKIKDDTGPVAKKPSSGK KGATTQNSEICSGQAPTPDQPDTSK RSK
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: TWNK twinkle mtDNA helicase [ Homo sapiens (human) ]
• Official Symbol: TWNK
• Synonyms: PEO; PEO1; SCA8; ATXN8; IOSCA; PEOA3; SANDO; TWINL; MTDPS7; PRLTS5; C10orf2
• Gene ID: 56652
• mRNA Refseq: NM_001163812
• Protein Refseq: NP_001157284
• UniProt ID: Q96RR1