Recombinant Human UBE2D2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: UBE2D2-5438H
• Product Overview: UBE2D2 MS Standard C13 and N15-labeled recombinant protein (NP_003330) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 16.7 kDa
• AA Sequence: MALKRIHKELNDLARDPPAQCSAGP VGDDMFHWQATIMGPNDSPYQGGVF FLTIHFPTDYPFKPPKVAFTTRIYH PNINSNGSICLDILRSQWSPALTIS KVLLSICSLLCDPNPDDPLVPEIAR IYKTDREKYNRIAREWTQKYAMTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: UBE2D2 ubiquitin-conjugating enzyme E2D 2 [ Homo sapiens (human) ]
• Official Symbol: UBE2D2
• Synonyms: UBE2D2; ubiquitin-conjugating enzyme E2D 2; ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5), ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2 D2; UBC4; UbcH5B; ubiquitin-protein ligase D2; ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2(17)KB 2; ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); PUBC1; UBC4/5; UBCH5B; E2(17)KB2
• Gene ID: 7322
• mRNA Refseq: NM_003339
• Protein Refseq: NP_003330
• MIM: 602962
• UniProt ID: P62837

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Q&As

How does UBE2D2 interact with other components of the ubiquitin-proteasome system?

UBE2D2 works in tandem with other ubiquitin-proteasome system components, orchestrating protein degradation.

How does UBE2D2 contribute to cellular protein degradation?

It facilitates the attachment of ubiquitin to target proteins, directing them to the proteasome for breakdown.

What role does UBE2D2 play in cell cycle regulation?

UBE2D2 plays a role in controlling the cell cycle by regulating the degradation of key cycle-related proteins.

What potential therapeutic applications arise from targeting UBE2D2 in diseases related to protein misfolding?

Targeting UBE2D2 could offer new treatments for conditions involving protein misfolding and aggregation.

What is the impact of altered UBE2D2 activity on disease development?

Altered UBE2D2 activity is linked with several diseases, including cancer and neurodegenerative disorders.

How do genetic variations in UBE2D2 affect cellular homeostasis?

Genetic mutations in UBE2D2 can disrupt protein turnover, affecting various cellular functions and stability.

What is the primary role of UBE2D2 in protein ubiquitination?

UBE2D2, a ubiquitin-conjugating enzyme, is vital for tagging proteins with ubiquitin, marking them for degradation.