Recombinant Human UBE3A Protein, C-Myc/DDK-Tagged

• Cat.No.: UBE3A-1699H
• Product Overview: Recombinant Human UBE3A Protein, transcript variant 3, C-Myc/DDK-Tagged was expressed in HEK293T cell

Specification

• Description: This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
• Source: HEK293T
• Species: Human
• Tag: C-Myc/DDK
• Molecular Mass: 99.9 kDa
• AA Sequence: MATACKRSGEPQSDDIEASRMKRAA AKHLIERYYHQLTEGCGNEACTNEF CASCPTFLRMDNNAAAIKAL
  ELYKINAKLCDPHPSKKGASSAYLE NSKGAPNNSCSEIKMNKKGARIDFK DVTYLTEEKVYEILELCRER
  EDYSPLIRVIGRVFSSAEALVQSFR KVKQHTKEELKSLQAKDEDKDEDEK EKAACSAAAMEEDSEASSSR
  IGDSSQGDNNLQKLGPDDVSVDIDA IRRVYTRLLSNEKIETAFLNALVYL SPNVECDLTYHNVYSRDPNY
  LNLFIIVMENRNLHSPEYLEMALPL FCKAMSKLPLAAQGKLIRLWSKYNA DQIRRMMETFQQLITYKVIS
  NEFNSRNLVNDDDAIVAASKCLKMV YYANVVGGEVDTNHNEEDDEEPIPE SSELTLQELLGEERRNKKGP
  RVDPLETELGVKTLDCRKPLIPFEE FINEPLNEVLEMDKDYTFFKVETEN KFSFMTCPFILNAVTKNLGL
  YYDNRIRMYSERRITVLYSLVQGQQ LNPYLRLKVRRDHIIDDALVRLEMI AMENPADLKKQLYVEFEGEQ
  GVDEGGVSKEFFQLVVEEIFNPDIG MFTYDESTKLFWFNPSSFETEGQFT LIGIVLGLAIYNNCILDVHF
  PMVVYRKLMGKKGTFRDLGDSHPVL YQSLKDLLEYEGNVEDDMMITFQIS QTDLFGNPMMYDLKENGDKI
  PITNENRKEFVNLYSDYILNKSVEK QFKAFRRGFHMVTNESPLKYLFRPE EIELLICGSRNLDFQALEET
  TEYDGGYTRDSVLIREFWEIVHSFT DEQKRLFLQFTTGTDRAPVGGLGKL KMIIAKNGPDTERLPTSHTC
  FNVLLLPEYSSKEKLKERLLKAITY AKGFGML
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80°C.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Cytogenetics: 15q11.2
• Refseq Size: 5211
• Refseq ORF: 2616
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: UBE3A ubiquitin protein ligase E3A [ Homo sapiens (human) ]
• Official Symbol: UBE3A
• Synonyms: ANCR; AS; E6-AP; EPVE6AP; HPVE6A
• Gene ID: 7337
• mRNA Refseq: NM_000462.5
• Protein Refseq: NP_570854
• MIM: 601623
• UniProt ID: Q05086