| Description : |
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
123 kDa |
| AA Sequence : |
MSVEAYGPSSQTLTFLDTEEAELLG ADTQGSEFEFTDFTLPSQTQTPPGG PGGPGGGGAGGPGGAGAGAAAGQLD AQVGPEGILQNGAVDDSVAKTSQLL AELNFEEDEEDTYYTKDLPIHACSY CGIHDPACVVYCNTSKKWFCNGRGN TSGSHIVNHLVRAKCKEVTLHKDGP LGETVLECYNCGCRNVFLLGFIPAK ADSVVVLLCRQPCASQSSLKDINWD SSQWQPLIQDRCFLSWLVKIPSEQE QLRARQITAQQINKLEELWKENPSA TLEDLEKPGVDEEPQHVLLRYEDAY QYQNIFGPLVKLEADYDKKLKESQT QDNITVRWDLGLNKKRIAYFTLPKT DSDMRLMQGDEICLRYKGDLAPLWK GIGHVIKVPDNYGDEIAIELRSSVG APVEVTHNFQVDFVWKSTSFDRMQS ALKTFAVDETSVSGYIYHKLLGHEV EDVIIKCQLPKRFTAQGLPDLNHSQ VYAVKTVLQRPLSLIQGPPGTGKTV TSATIVYHLARQGNGPVLVCAPSNI AVDQLTEKIHQTGLKVVRLCAKSRE AIDSPVSFLALHNQIRNMDSMPELQ KLQQLKDETGELSSADEKRYRALKR TAERELLMNADVICCTCVGAGDPRL AKMQFRSILIDESTQATEPECMVPV VLGAKQLILVGDHCQLGPVVMCKKA AKAGLSQSLFERLVVLGIRPIRLQV QYRMHPALSAFPSNIFYEGSLQNGV TAADRVKKGFDFQWPQPDKPMFFYV TQGQEEIASSGTSYLNRTEAANVEK ITTKLLKAGAKPDQIGIITPYEGQR SYLVQYMQFSGSLHTKLYQEVEIAS VDAFQGREKDFIILSCVRANEHQGI GFLNDPRRLNVALTRARYGVIIVGN PKALSKQPLWNHLLNYYKEQKVLVE GPLNNLRESLMQFSKPRKLVNTINP GARFMTTAMYDAREAIIPGSVYDRS SQGRPSSMYFQTHDQIGMISAGPSH VAAMNIPIPFNLVMPPMPPPGYFGQ ANGPAAGRGTPKGKTGRGGRQKNRF GLPGPSQTNLPNSQASQDVASQPFS QGALTQGYISMSQPSQMSQPGLSQP ELSQDSYLGDEFKSQIDVALSQDST YQGERAYQHGGVTGLSQYTRTRPLE QKLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
