Active Recombinant Human USP7 protein, His-tagged

• Cat.No.: USP7-490H
• Product Overview: Recombinant Human full length USP7(1-1102) fused with N terminal His tag was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: The activity of His-USP7 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysedcleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-USP7 was compared confirming the deubiquitylating activity of His-USP7.
• Molecular Mass: ~130 kDa
• AA Sequence: MGSSHHHHHHSSG LEVLFQGP G S M NHQQQQQQQKAGEQQLSEPEDMEME AGDTDDPPRITQNPVINGNVALSDG HNTAEEDMEDDTSWRSEATFQFTVE RFSRLSESVLSPPCFVRNLPWKIMV MPRFYPDRPHQKSVGFFLQCNAESD STSWSCHAQAVLKIINYRDDEKSFS RRISHLFFHKENDWGFSNFMAWSEV TDPEKGFIDDDKVTFEVFVQADAPH GVAWDSKKHTGYVGLKNQGATCYMN SLLQTLFFTNQLRKAVYMMPTEGDD SSKSVPLALQRVFYELQHSDKPVGT KKLTKSFGWETLDSFMQHDVQELCR VLLDNVENKMKGTCVEGTIPKLFRG KMVSYIQCKEVDYRSDRREDYYDIQ LSIKGKKNIFESFVDYVAVEQLDGD NKYDAGEHGLQEAEKGVKFLTLPPV LHLQLMRFMYDPQTDQNIKINDRFE FPEQLPLDEFLQKTDPKDPANYILH AVLVHSGDNHGGHYVVYLNPKGDGK WCKFDDDVVSRCTKEEAIEHNYGGH DDDLSVRHCTNAYMLVYIRESKLSE VLQAVTDHDIPQQLVERLQEEKRIE AQKRKERQEAHLYMQVQIVAEDQFC GHQGNDMYDEEKVKYTVFKVLKNSS LAEFVQSLSQTMGFPQDQIRLWPMQ ARSNGTKRPAMLDNEADGNKTMIEL SDNENPWTIFLETVDPELAASGATL PKFDKDHDVMLFLKMYDPKTRSLNY CGHIYTPISCKIRDLLPVMCDRAGF IQDTSLILYEEVKPNLTERIQDYDV SLDKALDELMDGDIIVFQKDDPEND NSELPTAKEYFRDLYHRVDVIFCDK TIPNDPGFVVTLSNRMNYFQVAKTV AQRLNTDPMLLQFFKSQGYRDGPGN PLRHNYEGTLRDLLQFFKPRQPKKL YYQQLKMKITDFENRRSFKCIWLNS QFREEEITLYPDKHGCVRDLLEECK KAVELGEKASGKLRLLEIVSYKIIG VHQEDELLECLSPATSRTFRIEEIP LDQVDIDKENEMLVTVAHFHKEVFG TFGIPFLLRIHQGEHFREVMKRIQS LLDIQEKEFEKFKFAIVMTGRHQYI NEDEYEVNLKDFEPQPGNMSHPRPW LGLDHFNKAPKRSRYTYLEKAIKIH N
• Purity: >80% by SDS-PAGE
• Stability: 12 months at -70 centigrade; aliquot as required
• Storage: Storage at -70 centigrade
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: USP7 ubiquitin specific peptidase 7 (herpes virus-associated) [ Homo sapiens ]
• Official Symbol: USP7
• Synonyms: USP7; ubiquitin specific peptidase 7 (herpes virus-associated); HAUSP, ubiquitin specific protease 7 (herpes virus associated); ubiquitin carboxyl-terminal hydrolase 7
• Gene ID: 7874
• mRNA Refseq: NM_003470
• Protein Refseq: NP_003461
• MIM: 602519
• UniProt ID: Q93009
• Chromosome Location: 16p13.3
• Pathway: FoxO family signaling, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; p53 pathway, organism-specific biosystem
• Function: cysteine-type endopeptidase activity; p53 binding; peptidase activity; protein C-terminus binding; protein binding

Reviews

10/22/2021

The product's widespread recognition among researchers made it a preferred choice for many scientific studies.

06/17/2018

Positive feedback from other labs and researchers confirmed its standing as a valuable research tool.

06/28/2016

The product's respected reputation in the scientific community was a testament to its quality and effectiveness.

Q&As

Does USP7 interact with specific protein substrates?

Yes, USP7 interacts with various protein substrates, including p53, MDM2, and PTEN, influencing their stability and cellular functions.

Can USP7 inhibitors be potential cancer therapeutics?

Inhibitors targeting USP7 are being explored as potential cancer therapeutics, aiming to disrupt the stability of oncogenic proteins and inhibit cancer cell growth.

Does USP7 have tissue-specific expression patterns?

USP7 exhibits tissue-specific expression patterns, and its levels may vary in different tissues and cell types.

What is the significance of USP7 in neurodegenerative diseases?

USP7 has been implicated in neurodegenerative diseases, and its dysregulation may contribute to protein aggregation and neuronal dysfunction.

Are there post-translational modifications of USP7?

Yes, USP7 undergoes post-translational modifications, including phosphorylation and ubiquitination, which can regulate its activity and stability.

How does USP7 regulate the p53 pathway?

USP7 regulates the p53 pathway by deubiquitinating and stabilizing p53, preventing its degradation and promoting its transcriptional activity.

Is USP7 involved in the immune response?

Yes, USP7 plays a role in the immune response by regulating the stability of proteins involved in immune signaling pathways.