Recombinant Human VEGFA protein, His-tagged
Cat.No. : | VEGFA-407H |
Product Overview : | Recombinant Human VEGFA protein (Ala27-Arg147), fused to His tag at N-terminus, was expressed in human 293 cells (HEK293). |
- Specification
- Gene Information
- Related Products
Description : | Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C. |
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
Molecular Mass : | The protein has a calculated MW of 15.0 kDa. As a result of glycosylation, the protein migrates as 17 kDa and 20 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE). |
Protein length : | 121 |
Endotoxin : | Less than 1.0 EU per ug by the LAL method. |
Purity : | >95% as determined by reduced SDS-PAGE. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 centigrade to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | VEGFA |
Official Symbol : | VEGFA |
Synonyms : | VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609 |
Gene ID : | 7422 |
mRNA Refseq : | NM_001025366 |
Protein Refseq : | NP_001020537 |
MIM : | 192240 |
UniProt ID : | P15692 |
Products Types
◆ Recombinant Protein | ||
VEGF165-12H | Active Recombinant Human VEGF165 Protein | +Inquiry |
VEGFA-260H | Active Recombinant Human VEGF-121 Protein | +Inquiry |
Vegfa-146V | Active Recombinant Mouse Vegf164 Protein (165 aa) | +Inquiry |
VEGFA-259E | Active Recombinant Equine VEGF-165 Protein | +Inquiry |
VEGFA-561H | Recombinant Human VEGFA Protein, His-tagged | +Inquiry |
◆ Native Protein | ||
VEGFA-31701TH | Native Human VEGFA | +Inquiry |
◆ Lysates | ||
VEGFA-522RCL | Recombinant Rat VEGFA cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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