Recombinant Human XRCC5&XRCC6 protein(Met1-Ile732 & Met1-Asp609), His & His-tagged

• Cat.No.: XRCC5&XRCC6-1340H
• Product Overview: Recombinant XRCC5 (P13010) (Met 1-Ile 732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; Recombinant XRCC6 (P12956) (Met 1-Asp 609) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified and expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His & His
• Protein length: Met1-Ile732 & Met1-Asp609
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of Human XRCC5/XRCC5 comprises 1379 (751 + 628) amino acids and has a calculated molecular mass of 157 (85 + 72) kDa. The apparent molecular mass of rh XRCC5/XRCC5 heterodimer is approximately 70 & 85 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) [ Homo sapiens ]
• Official Symbol: XRCC5; XRCC6
• Synonyms: XRCC5; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing protein 5; KARP 1; Ku autoantigen; 80kDa; KU80; Ku86; KUB2; TLAA; CTC85; CTCBF; nuclear factor IV; Ku autoantigen, 80kDa; DNA repair protein XRCC5; thyroid-lupus autoantigen; 86 kDa subunit of Ku antigen; lupus Ku autoantigen protein p86; Ku86 autoantigen related protein 1; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; NFIV; KARP1; KARP-1; FLJ39089
• Gene ID: 7520
• mRNA Refseq: NM_021141
• Protein Refseq: NP_066964
• MIM: 194364
• UniProt ID: P13010

Reviews

01/25/2023

The combination of high-quality XRCC5 protein and excellent technical support provided by the manufacturer makes them the ideal choice for my research.

09/09/2019

The manufacturer's extensive experience and expertise in the field of XRCC5 protein research make them a trusted partner.

08/03/2019

The manufacturer's commitment to providing excellent technical support is highly valuable.

Q&As

How does XRCC5 function in DNA repair?

XRCC5 forms a heterodimer with XRCC6 (Ku70) and plays a crucial role in non-homologous end joining (NHEJ) repair of DNA double-strand breaks.

What are the clinical implications of XRCC5 mutations?

Mutations in XRCC5 have been associated with an increased risk of cancer and may affect the response to certain cancer treatments.

Are there diagnostic tests to detect XRCC5 mutations?

Yes, genetic testing can identify XRCC5 mutations, which can be important for assessing cancer risk.

Can XRCC5 mutations lead to cancer susceptibility?

Yes, mutations in XRCC5 have been linked to an increased susceptibility to certain types of cancer, such as breast and ovarian cancer.

How can XRCC5 be targeted in cancer therapy?

XRCC5 inhibitors are being investigated as potential cancer therapeutics to sensitize cancer cells to radiation and chemotherapy.