Recombinant Influenza [A/Shanghai/2/2013(H7N9)] Hemagglutinin (HA) protein, Fc-tagged

• Cat.No.: HA-354I
• Product Overview: Recombinant Influenza [A/Shanghai/2/2013(H7N9)] Hemagglutinin (HA) protein (Asp19-Gly338, Leu341-Asp523), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: Influenza, commonly known as "the flu", is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. The type A viruses are the most virulent human pathogens among the three influenza types and cause the most severe disease. The serotypes that have been confirmed in humans, ordered by the number of known human pandemic deaths, are: H1N1, H2N2, H3N2, H5N1, H7N7, H1N2, H9N2, H7N2, H7N3, H10N7, H7N9. H7N9 is a serotype of the species Influenzavirus A (avian influenza virus or bird flu virus). H7 normally circulates amongst avian populations with some variants known to occasionally infect humans. An H7N9 virus was first reported to have infected humans in 2013 in China.
• Source: HEK293
• Species: Influenza [A/Shanghai/2/2013(H7N9)]
• Tag: human IgG1 Fc
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 84.5 kDa. The protein migrates as 110-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 503
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: HA
• Official Symbol: HA
• Synonyms: TS66_s4gp1
• Gene ID: 23104227
• Protein Refseq: YP_009118475.1
• UniProt ID: R4NN21

Reviews

06/01/2021

The transport process does not affect the protein quality, and the stability is good.

11/27/2019

Higher solubility makes it easy to prepare the desired concentration.

Q&As

How about the structural characteristics of HA protein?

HA protein is a glycoprotein that contains thousands of carbohydrates and an amino acid chain consisting of 566 amino acid residues. The structure of HA protein has triple symmetry, consisting of three monomers that form a spiral.

Will genetic variants in HA proteins affect vaccine development?

Genetic variants in the HA protein may affect the development and production of influenza vaccines. Because HA protein is an important component of influenza viruses, different subtypes or variants of HA protein may require different vaccines to prepare.

Why is hemagglutinin protein related to viral replication?

This protein is one of the key factors in the replication process of influenza viruses. When a virus infects a host cell, the receptor-binding region of the HA protein binds to the receptor on the cell surface, allowing the virus to invade the host cell and replicate.

What are the subtypes of HA protein?

There are currently 18 known HA subtypes of influenza viruses, H1-H18. Among them, the H1, H2, H3, H5, H7, and H9 subtypes are associated with human viruses, while other subtypes are generally associated with animal viruses.

What are the physiological functions of HA protein?

The main physiological function of HA protein is to bind to host cells and mediate the entry of influenza viruses into host cells. It contains specific regions that bind glycoprotein receptors that allow the influenza virus to adhere to them. This is an important link that allows the virus to stably bind and enter the cell.

What laboratory techniques are HA proteins used in?

HA protein is widely used in viral research and drug development. Researchers can use HA proteins to test drugs such as influenza virus activators, antibodies and vaccines, and HA proteins are also widely used to develop novel immunoassays and treatments.