Recombinant Mouse Abcb4 protein, His & T7-tagged
Cat.No. : | Abcb4-8124M |
Product Overview : | Recombinant Mouse Abcb4 aa. (Asn1034~Leu1276 (Accession # P21440)) fused with N-terminal His & T7 tag was produced in E. coli cells. |
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Source : | E. coli |
Species : | Mouse |
Tag : | His & T7 |
Form : | Freeze-dried powder |
Molecular Mass : | Predicted Molecular Mass: 30.3kDa. |
Protein length : | Asn1034~Leu1276 (Accession # P21440) |
Endotoxin : | <1.0EU per 1ug (determined by the LAL method) |
Purity : | >95% |
Characteristic : | The isoelectric point is 7.9. |
Applications : | SDS-PAGE; WB; ELISA; IP. |
Stability : | The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage Buffer : | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
Reconstitution : | Reconstitute in sterile ddH2O. |
Gene Name : | Abcb4 ATP-binding cassette, sub-family B (MDR/TAP), member 4 [ Mus musculus (house mouse) ] |
Official Symbol : | Abcb4 |
Gene ID : | 18670 |
mRNA Refseq : | NM_008830.2 |
Protein Refseq : | NP_032856.2 |
UniProt ID : | P21440 |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThe endotoxin level of recombinant protein can be customized according to customer needs, and you can communicate with our technical experts in detail.
We provide these two types of recombinant proteins, you can buy or customize according to your needs.
We provide recombinant protein products with different purities, and you can communicate with our technical experts about your customized needs.
Both have their own advantages, and the freeze-dried form is more stable than the liquid form, but the freeze-drying process may cause certain damage to the structure and activity of the protein.
Bile contains three main lipids: cholesterol, phospholipids and bile acids. ABCB4 protein participates in phospholipid transport and is crucial for the formation of bile.
ABCB4 protein is also known as multidrug resistance protein 3 (MDR3).
It is generally recommended to incubate at room temperature for about two hours, but the exact time may also vary.
Quantification can be performed in the linear range of detection using ImageJ software.
If you have requirements for the product format, please note when placing an order.
You can use the Uptima bicinchoninic acid protein assay to determine the protein concentrations.
Customer Reviews (2)
Write a reviewI purchased this protein product for Western blotting, and the feedback results are not bad, and it can meet my experimental needs.
I have received my customized recombinant ABCB4 protein, and the delivery cycle is short
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