Recombinant Mouse Acaa1a protein, His & T7-tagged

• Cat.No.: Acaa1a-8154M
• Product Overview: Recombinant Mouse Acaa1a aa. (Gly182~Asn424 (Accession # Q921H8)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.9kDa.
• Protein length: Gly182~Asn424 (Accession # Q921H8)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: Acaa1a acetyl-Coenzyme A acyltransferase 1A [ Mus musculus (house mouse) ]
• Official Symbol: Acaa1a
• Gene ID: 113868
• mRNA Refseq: NM_130864.3
• Protein Refseq: NP_570934.1
• UniProt ID: Q921H8

Reviews

11/25/2019

Very sensitive in flow cytometry analysis, accurately detecting low expression levels of proteins.

11/28/2018

Performs outstandingly in protein purification, efficiently purifying target proteins.

Q&As

What is the function of acetyl-Coenzyme A acyltransferase 1A protein?

The function of acetyl-Coenzyme A acyltransferase 1A protein is to catalyze the transfer of an acyl group from acyl-CoA to CoA, producing acetyl-CoA.

What is the tissue distribution of acetyl-Coenzyme A acyltransferase 1A expression?

Acetyl-Coenzyme A acyltransferase 1A is expressed in various tissues, including liver, muscle, and adipose tissue.

What is the evolutionary history of the acetyl-Coenzyme A acyltransferase 1A gene and protein?

The acetyl-Coenzyme A acyltransferase 1A gene and protein have been conserved throughout evolution and are found in a wide range of organisms, from bacteria to humans.

What are the substrates of acetyl-Coenzyme A acyltransferase 1A?

The substrates of acetyl-Coenzyme A acyltransferase 1A are acyl-CoA molecules of varying chain lengths.

How is acetyl-Coenzyme A acyltransferase 1A regulated?

Acetyl-Coenzyme A acyltransferase 1A is regulated by post-translational modifications, such as phosphorylation, and by transcriptional regulation through various signaling pathways.

What is the role of acetyl-Coenzyme A acyltransferase 1A in metabolism?

Acetyl-Coenzyme A acyltransferase 1A plays a key role in fatty acid metabolism and the production of acetyl-CoA, which is an important molecule in energy production.

How is the activity of acetyl-Coenzyme A acyltransferase 1A affected by cellular conditions such as pH or temperature?

The activity of acetyl-Coenzyme A acyltransferase 1A is affected by various cellular conditions, including pH and temperature.

What is the structure of the acetyl-Coenzyme A acyltransferase 1A protein?

The structure of acetyl-Coenzyme A acyltransferase 1A protein consists of a homodimeric complex with each monomer consisting of several domains.

What is the relationship between acetyl-Coenzyme A acyltransferase 1A and fatty acid metabolism?

Acetyl-Coenzyme A acyltransferase 1A is a key enzyme in the beta-oxidation pathway of fatty acid metabolism, producing acetyl-CoA for use in the citric acid cycle.

What diseases are associated with mutations in the acetyl-Coenzyme A acyltransferase 1A gene?

Mutations in the acetyl-Coenzyme A acyltransferase 1A gene are associated with various metabolic disorders, including fatty acid oxidation disorders.