Recombinant Mouse Acbd4 Protein, Myc/DDK-tagged

• Cat.No.: Acbd4-1490M
• Product Overview: Purified recombinant protein of mouse full-length acyl-Coenzyme A binding domain containing 4 (Acbd4), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Broad expression in duodenum adult (RPKM 33.3), subcutaneous fat pad adult (RPKM 30.6) and 23 other tissues
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 37.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Acbd4 acyl-Coenzyme A binding domain containing 4 [ Mus musculus (house mouse) ]
• Official Symbol: Acbd4
• Synonyms: ACBD4; acyl-Coenzyme A binding domain containing 4; AI849317; 2010009P05Rik; 2010015A21Rik
• Gene ID: 67131
• mRNA Refseq: NM_025988
• Protein Refseq: NP_080264
• UniProt ID: Q80X94

Reviews

09/16/2022

I saved a lot of time and effort by purchasing the recombinant human ACBD4 protein from Creative BioMart instead of making it myself.

02/14/2021

The recombinant mouse ACBD4 protein from Creative BioMart was reasonably priced and saved me money compared to other suppliers.

10/07/2020

I am very satisfied with the quality and performance of the Recombinant Zebrafish ACBD4. It met all of our research needs.

Q&As

What is the role of ACBD4 in the development of metabolic diseases?

Studies have shown that ACBD4 expression is altered in metabolic diseases such as obesity and diabetes. ACBD4 knockout mice exhibit reduced body weight, improved glucose tolerance, and reduced hepatic steatosis, suggesting that ACBD4 may be a potential target for the treatment of metabolic diseases.

What is the stability of the recombinant human ACBD4 protein under different storage conditions?

It is generally stable when stored at -20°C or -80°C for up to six months to two years, respectively. However, the protein may be sensitive to freeze-thaw cycles, and repeated freeze-thaw cycles should be avoided. It is also recommended to aliquot the protein upon arrival to minimize the number of freeze-thaw cycles.

Can the recombinant Rat ACBD4 protein be used in functional assays?

Yes, it can be used in a variety of functional assays, such as enzyme assays, binding assays, and activity assays, to study its biochemical and biophysical properties.

What is the recommended concentration of the Recombinant Human ACBD4 Protein (ACBD4-148H) for use in experiments?

It may vary depending on the application and the specific experimental conditions. Typically, a concentration of 0.1-1 mg/mL is sufficient for most assays, but this may need to be optimized based on the experimental setup. It is recommended to use a range of concentrations to determine the optimal concentration for a specific experiment.

What is the recommended concentration of recombinant ACBD4 protein for research studies?

It varies depending on the specific application. It is important to verify the appropriate concentration for each specific application in order to ensure optimal results.

What is the concentration of endotoxin in the recombinant human ACBD4 protein?

The endotoxin concentration is typically below 1.0 eu/μg of protein, as determined by a Limulus Amebocyte Lysate (LAL) assay. In addition, you can choose the concentration you need when submitting the inquiry form.

Do you sell polyclonal antibodies against human ACBD4?

You can describe your experimental needs in detail to our scientists, such as WB, IF, IP, IHC, and they will provide professional solutions.

What is the proper way to store the recombinant ACBD4 protein?

It should be stored at -20°C or -80°C in a non-frost free freezer. It is important to avoid repeated freeze-thaw cycles in order to prevent degradation of the protein. It is recommended that the protein be aliquoted into smaller portions to avoid contamination from repeated use.

What is the structure of ACBD4 protein?

ACBD4 protein contains an N-terminal acyl-CoA binding domain that binds to long-chain acyl-CoA esters and a C-terminal domain that interacts with the Golgi membrane. It also contains a hydrophobic loop that plays a crucial role in the binding of ACBD4 to the Golgi membrane.

What are the signaling pathways involved in the regulation of ACBD4 expression?

Several signaling pathways, including the AMP-activated protein kinase (AMPK) and the mammalian target of rapamycin (mTOR) pathways, have been shown to regulate ACBD4 expression.