Recombinant Mouse Acp5 protein, His & T7-tagged

• Cat.No.: Acp5-7904M
• Product Overview: Recombinant Mouse Acp5 aa. (Leu28~Gly286 (Accession # Q05117)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.1kDa
• Protein length: Leu28~Gly286 (Accession # Q05117)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.2.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Acp5 acid phosphatase 5, tartrate resistant [ Mus musculus (house mouse) ]
• Official Symbol: Acp5
• Synonyms: TRAP; TRACP; TR-AP; acid phosphatase type V; tartrate-resistant acid ATPase; trATPase; type 5 acid phosphatase; tartrate-resistant acid phosphatase type 5
• Gene ID: 11433
• mRNA Refseq: NM_001102404.1
• Protein Refseq: NP_001095874.1
• UniProt ID: Q05117

Reviews

08/03/2022

Arrived prompt - good service

09/11/2021

Much better than similar products that I've used.

07/02/2021

Used in protein electron microscopy structure analysis.Great.

01/10/2021

Great performance in WB :)

06/20/2018

Patient customer service, love it.

Q&As

What will happen if loss ACP5 in vivo?

the loss of Acp5 significantly protected mice from BLM-induced lung injury and fibrosis, accompanied by a marked reduction in myofibroblast accumulation.

Could please list three drugs for ACP5 gene?

Alteplase, Filgrastim, and Streptozocin.

What are four reactome pathways for ACP5 gene?

They are Metabolism; Metabolism of vitamins and cofactors; Metabolism of water-soluble vitamins and cofactors; Vitamin B2 (riboflavin) metabolism

How does ACP5 epithelial-mesenchymal transition (EMT)?

ACP5 promoted A549 and NCI-H1975 cells undergoing epithelial-mesenchymal transition (EMT) via P53/Smad3 signaling.

Can you briefly descibe the molecular function of ACP5 protein?

Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.

How about the human health condition related to ACP5 gene changes?

Diseases associated with ACP5 include spondyloenchondrodysplasia with Immune dysregulation and Hairy Cell Leukemia. Among its related pathways are metabolism of water-soluble. vitamins and cofactors and Metabolism

What is the effect of ACP5 serum concentration on Idiopathic pulmonary fibrosis (IPF) patients?

Serum concentration of ACP5 was likely associated with decreased lung function in IPF patients.

What is the role of ACP5 on the process of fibroblast's cell differentiation and proliferation?

Acp5 is upregulated by transforming growth factor-β1 (TGF-β1) in a TGF-β receptor 1 (TGFβR1)/Smad family member 3 (Smad3)-dependent manner, after which Acp5 dephosphorylates p-β-catenin at serine 33 and threonine 41, inhibiting the degradation of β-catenin and subsequently enhancing β-catenin signaling in the nucleus, which promotes the differentiation, proliferation and migration of fibroblast.

What functions does ACP5 could apply when we act it as biomarks?

ACP5 is evaluated as osteoclast differentiation/activity markers in serum and in culture.

What will happen to mice if ACP5 deficiency present?

Acp5 deficiency protects mice from BLM-induced lung injury and fibrosis coupled with a significant reduction of fibroblast differentiation and proliferation.