Recombinant Mouse Acsl3 Protein, His-tagged

• Cat.No.: Acsl3-54M
• Product Overview: Recombinant Mouse Acsl3 Protein (Cys450-Lys720) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable arachidonate-CoA ligase activity; protein domain specific binding activity; and protein kinase binding activity. Predicted to be involved in several processes, including fatty acid metabolic process; positive regulation of phosphatidylcholine biosynthetic process; and positive regulation of transport. Predicted to act upstream of or within fatty acid metabolic process. Predicted to be located in Golgi apparatus and perinuclear region of cytoplasm. Predicted to be active in endoplasmic reticulum; lipid droplet; and plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in asthma; breast cancer; and prostate cancer. Orthologous to human ACSL3 (acyl-CoA synthetase long chain family member 3).
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34 kDa
  Accurate Molecular Mass: 35 kDa
• Protein length: Cys450-Lys720
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Acsl3 acyl-CoA synthetase long-chain family member 3 [ Mus musculus (house mouse) ]
• Official Symbol: Acsl3
• Synonyms: ACSL3; acyl-CoA synthetase long-chain family member 3; long-chain-fatty-acid--CoA ligase 3; LACS 3; long-chain acyl-CoA synthetase 3; fatty acid Coenzyme A ligase, long chain 3; Acs3; Facl3; C85929; Pro2194; 2610510B12Rik
• Gene ID: 74205
• mRNA Refseq: NM_001033606
• Protein Refseq: NP_001028778
• UniProt ID: Q9CZW4

Reviews

06/14/2021

The product was well-characterized, and the documentation provided was thorough and helpful.

08/27/2020

The protein arrived quickly and was exactly what I needed for my experiment. Very satisfied with my purchase.

06/01/2020

The website was easy to navigate and the ordering process was simple. Appreciate the user-friendly interface.

10/04/2019

I'm really pleased with my purchase of the recombinant protein. It's high quality and reasonably priced.

09/01/2018

I appreciate the transparency and honesty of this company in their communication with customers. Will continue to order from them in the future.

Q&As

What is the relationship between ACSL3 and cardiovascular disease?

ACSL3 dysregulation has been linked to the development of cardiovascular disease, highlighting the importance of the gene in the regulation of lipid metabolism and cardiovascular health.

What is the impact of ACSL3 on autophagy?

ACSL3 has been shown to impact autophagy, a cellular process involved in the breakdown and recycling of damaged or dysfunctional cellular components.

What is the relationship between ACSL3 and insulin resistance?

ACSL3 dysregulation has been implicated in the development of insulin resistance, a common feature of many metabolic disorders.

What is the impact of ACSL3 on adipogenesis?

ACSL3 has been shown to impact adipogenesis, the process of fat cell differentiation, by regulating the availability of long-chain fatty acids for lipid synthesis and storage.

What is the structure of the ACSL3 protein?

The ACSL3 protein consists of multiple domains, including an N-terminal domain, a central domain, and a C-terminal domain, which are involved in substrate binding, acyl-adenylate formation, and ATP binding, respectively.

What is the impact of ACSL3 on cellular signaling pathways?

ACSL3 has been shown to impact cellular signaling pathways, particularly those involved in the regulation of inflammation, insulin signaling, and energy metabolism.

What is the role of ACSL3 in lipid metabolism?

ACSL3 plays a critical role in lipid metabolism by activating long-chain fatty acids for subsequent utilization in various metabolic pathways, including β-oxidation and the production of signaling lipids.

What is the expression pattern of ACSL3 in different tissues?

ACSL3 is expressed in a variety of tissues, including adipose tissue, liver, muscle, heart, and brain, with highest expression levels observed in adipose tissue.

How does ACSL3 impact lipid droplet formation?

ACSL3 has been shown to impact lipid droplet formation, with increased expression of the gene leading to enhanced lipid droplet formation and storage.

How does ACSL3 impact cellular proliferation and differentiation?

ACSL3 has been shown to impact cellular proliferation and differentiation by modulating the availability of long-chain fatty acids for various cellular processes.