Recombinant Mouse Add1 Protein, His-tagged

• Cat.No.: Add1-492M
• Product Overview: Recombinant Mouse Add1(Gly388~Gly560) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 23.0kDa
• Protein length: Gly388~Gly560
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: Add1 adducin 1 (alpha) [ Mus musculus ]
• Official Symbol: Add1
• Synonyms: ADD1; adducin 1 (alpha); alpha-adducin; erythrocyte adducin subunit alpha; AI256389
• Gene ID: 11518
• mRNA Refseq: NM_001024458
• Protein Refseq: NP_001019629
• UniProt ID: Q9QYC0

Reviews

01/01/2019

Highly effective in detecting and quantifying the expression levels of fusion proteins in protein expression analysis.

06/09/2017

Highly specific in detecting the target protein in tissue sections in immunohistochemistry experiments.

11/01/2016

Demonstrates high selectivity and sensitivity in detecting protein-protein interactions in pull-down assays.

Q&As

Can we develop specific antibodies or probes to detect and visualize the localization of ADD1 in live cells or tissues?

Specific antibodies or probes can be developed to visualize the localization of ADD1 in live cells or tissues.

What is the impact of altering the expression level or activity of ADD1 on cellular processes such as cell proliferation, differentiation, or apoptosis?

Altering the expression level or activity of ADD1 can affect cellular processes like cell proliferation, differentiation, or apoptosis.

How does the expression of ADD1 change under various physiological or pathological conditions?

The expression of ADD1 can change under different physiological or pathological conditions.

What is the subcellular localization of ADD1 within the cell? Is it predominantly found in the nucleus, cytoplasm, or other cellular compartments?

ADD1 exhibits subcellular localization in various cellular compartments, such as the nucleus or cytoplasm.

What are the interacting partners or binding proteins of ADD1 in a specific cellular context?

ADD1 interacts with specific binding proteins or partners in a context-dependent manner.

What is the tissue-specific expression pattern of ADD1 in different organs or cell types?

The tissue-specific expression pattern of ADD1 varies across organs and cell types.

Are there any post-translational modifications (e.g., phosphorylation, acetylation) that regulate the activity or stability of ADD1?

ADD1 undergoes post-translational modifications, including phosphorylation and acetylation, which can impact its activity and stability.

Are there any small molecule inhibitors or activators that can modulate the function of ADD1? What is their mechanism of action?

There may exist small molecule inhibitors or activators that can modulate ADD1's function, with distinct mechanisms of action.

Can we identify the specific domains or motifs within ADD1 that are responsible for its functional activities?

Specific domains or motifs within ADD1 are responsible for its functional activities.

What are the downstream target genes or signaling pathways regulated by ADD1, and how do they contribute to its biological functions?

ADD1 regulates downstream target genes or signaling pathways that contribute to its biological functions.