Recombinant Mouse Add3 Protein, Myc/DDK-tagged

• Cat.No.: Add3-1537M
• Product Overview: Purified recombinant protein of mouse full-length adducin 3 (gamma) (Add3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping. Binds to calmodulin.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 78.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Add3 adducin 3 (gamma) [ Mus musculus (house mouse) ]
• Official Symbol: Add3
• Synonyms: Add3; adducin 3 (gamma); R75380; AI463285; gamma-adducin; adducin-like protein 70; cytoskeletal protein
• Gene ID: 27360
• mRNA Refseq: NM_013758
• Protein Refseq: NP_038786
• UniProt ID: Q9QYB5

Reviews

03/06/2021

Shows excellent performance in proximity ligation assays for protein-protein interaction studies.

07/25/2020

Performs exceptionally well in protein crystallization experiments, improving crystal quality and yield.

Q&As

What are the consequences of modulating the expression or activity of ADD3 on cellular processes like cell adhesion, cytoskeletal organization, or membrane dynamics?

Modulating the expression or activity of ADD3 can impact cellular processes like cell adhesion, cytoskeletal organization, or membrane dynamics.

What are the downstream signaling pathways or target molecules regulated by ADD3, and how do they contribute to its cellular functions?

ADD3 regulates downstream signaling pathways or target molecules that contribute to its cellular functions.

What is the subcellular localization of ADD3 within cells? Does it exhibit any dynamic localization or translocation?

ADD3 exhibits subcellular localization, and its localization may undergo dynamic changes or translocation.

Can the functional domains or motifs within ADD3 be identified and characterized?

Functional domains or motifs within ADD3 can be identified and characterized.

What post-translational modifications, such as phosphorylation or acetylation, occur on ADD3 and how do they impact its function?

Post-translational modifications, such as phosphorylation or acetylation, occur on ADD3 and can influence its function.

Are there specific interacting proteins or binding partners that associate with ADD3 in a cell- or context-specific manner?

ADD3 interacts with specific proteins or binding partners, and these interactions can be context-dependent.

How is the expression of ADD3 regulated during development or in response to specific stimuli?

The expression of ADD3 can be regulated during development or in response to specific stimuli.

Can specific antibodies or probes be developed to visualize the localization or dynamics of ADD3 in live cells or tissues?

Specific antibodies or probes can be developed to visualize the localization or dynamics of ADD3 in live cells or tissues.

Are there any small molecules or drugs that can selectively modulate the activity or stability of ADD3?

There may exist small molecules or drugs that selectively modulate the activity or stability of ADD3.

What is the expression pattern of ADD3 in different tissues and cell types?

The expression pattern of ADD3 varies across different tissues and cell types.