Recombinant mouse Alox12 Protein, His-tagged

• Cat.No.: Alox12-021M
• Product Overview: Recombinant mouse Alox12 protein (2-664) with a C-terminal His-tag was expressed in insect cells

Specification

• Description: This gene is from arachidonic acid. There are two main types of 12-LO, platelet-type and leukocyte-type, which share only 60% identity at the amino acid level and strongly differ in substrate specificity and enzyme kinetics. Leukocyte-type 12-LO has the ability to also produce 15-HpETE, however, platelet-type predominately forms 12-HpETE. 2 Epidermal-type 12(S)- and 12(R)-LO enzymes have also been characterized, which catalyze the synthesis of their respective stereospecific 12-HETE products.
• Source: Insect Cells
• Species: Mouse
• Tag: His
• Molecular Mass: 76 kDa
• Protein length: 2-664
• Purity: 80% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to consume 1 nmol of oxygen per minute at 37 centigrade in 0.1 M Tris-HCl, pH 7.5, containing 5 mM EDTA, 0.03% polysorbate, and 100 µM arachidonate.
• Stability: ≥ 1 year
• Storage: -80 centigrade
• Storage Buffer: 50 mM sodium phosphate pH 7.2, 100 mM sodium chloride, 20 µM FeCl2, and 30% glycerol
• Shipping: Dry ice in continental US, may vary elsewhere.

Gene Information

• Gene Name: Alox12 arachidonate 12-lipoxygenase [ Mus musculus (house mouse) ]
• Official Symbol: Alox12
• Synonyms: Alox12; arachidonate 12-lipoxygenase; P-12LO; Alox12p; 9930022G08Rik; polyunsaturated fatty acid lipoxygenase ALOX12; 12S-LOX; 12S-lipoxygenase; arachidonate (12S)-lipoxygenase; arachidonate (15S)-lipoxygenase; arachidonate 12-lipoxygenase, 12S-type; arachidonate 15-lipoxygenase,15S-type; linoleate 13S-lipoxygenase; lipoxin synthase 12-LO; platelet-type lipoxygenase 12; EC 1.13.11.31; EC 1.13.11.33
• Gene ID: 11684
• mRNA Refseq: NM_001331118
• Protein Refseq: NP_001318047
• UniProt ID: P39655