Recombinant Mouse Apex2 Protein, Myc/DDK-tagged

• Cat.No.: Apex2-1659M
• Product Overview: Purified recombinant protein of mouse full-length apurinic/apyrimidinic endonuclease 2 (Apex2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Functions as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 57.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Apex2 apurinic/apyrimidinic endonuclease 2 [ Mus musculus (house mouse) ]
• Official Symbol: Apex2
• Synonyms: APEX2; apurinic/apyrimidinic endonuclease 2; DNA-(apurinic or apyrimidinic site) lyase 2; APEX nuclease 2; AP endonuclease 2; apurinic-apyrimidinic endonuclease 2; ape2; C430040P13Rik
• Gene ID: 77622
• mRNA Refseq: NM_029943
• Protein Refseq: NP_084219
• UniProt ID: Q68G58

Reviews

11/08/2021

APEX2 produced by them has high expression and easy detection.

03/29/2021

Maximizes the use of energy and resources by optimizing the production process and equipment.

05/23/2020

The results are very consistent in each experiment or application, thanks to its high stability and reproducibility, which provides great convenience for follow-up studies.

Q&As

What is the detection principle of APEX2 ?

The APEX2 enables the detection of proteins by producing oxidation products and forming a stain in a visible range.

What are the advantages of APEX2 s compared with other labeling techniques?

Recombinant APEX2 proteins are more sensitive and stable than other labeling technologies, allowing for labeling and detection in a shorter time.

What are the advantages of APEX2 ?

APEX2 has the characteristics of high sensitivity, high specificity and rapid response, which is suitable for a variety of research and therapeutic needs.

How to prepare APEX2 ?

APEX2 can be expressed and purified by genetic engineering technology.

What is the mechanism of action of APEX2 ?

The APEX2 applies detectable markers to the protein through oxidation reactions, enabling visual study of the target protein.

What is the research progress of APEX2 in cancer field?

Recombinant APEX2 protein can be used as a tumor marker to help realize early diagnosis, treatment monitoring and prognosis assessment of cancer.

How to evaluate the effect of APEX2 ?

The effect of APEX2 s can usually be evaluated by comparing markers such as labeling effect, specificity, and sensitivity.

What are the limitations of APEX2 ?

The recombinant APEX2 protein may have errors under certain conditions due to its special oxidation reaction mechanism.

In addition to the field of cancer, does APEX2 have applications in other disease research?

Yes, APEX2 also has application potential in neurodegenerative diseases, cardiovascular diseases and other fields.

What are the clinical applications of APEX2 ?

The recombinant APEX2 protein can be used clinically in the fields of labeling targeted drugs, tumor therapy and biosensors.